CAJ | 학술논문

目的：观察裴氏升血颗粒（PG）含药血清对人白血病细胞株K562/ADR细胞p170蛋白表达水平的影响。方法：采用MTT法测定不同浓度含药血清对K562细胞系的细胞毒作用，用流式细胞仪检测非细胞毒性浓度含药血清处理后K562/ADR细胞膜表面p170表达变化。结果：不同浓度含药血清对K562/ADR细胞无明显细胞毒作用，非细胞毒性含药血清能明显下调K562/ADR细胞p170蛋白的表达。结论：裴氏升血颗粒能够提高化疗疗效，其机制可能与下调p170蛋白表达有关。
목적：관찰배씨승혈과립（PG）함약혈청대인백혈병세포주K562/ADR세포p170단백표체수평적영향。방법：채용MTT법측정불동농도함약혈청대K562세포계적세포독작용，용류식세포의검측비세포독성농도함약혈청처리후K562/ADR세포막표면p170표체변화。결과：불동농도함약혈청대K562/ADR세포무명현세포독작용，비세포독성함약혈청능명현하조K562/ADR세포p170단백적표체。결론：배씨승혈과립능구제고화료료효，기궤제가능여하조p170단백표체유관。
Objective:To investigate the effect of PG herbal serum to the expression level of p170 protein on the human leukemia K562/ADR cells. Methods: Testing the cytotoxic effect of different herbal serum to K562/ADR cells line by MTT method, using Flow cytometry to determine the expressive and functional changes of p170 on K562/ADR cells membrane dealed with the herbal serum at non-cytotoxic concentration. Results:Different herbal serum had no obvious cytotoxic effect to K562/A02 cells, non-cytotoxic concentration of herbal serum can significantly lowered the expression of p170 protein on the K562/ADR cells. Conclusion: PG could improve the curative effect of chemotherapy, and its mechanism may be related to descend the expression of p170.