CAJ | 학술논문

目的探讨唾液乳杆菌对哮喘Balb/c小鼠血清及肺组织匀浆中IgE、IL-4及IFN-γ的影响.方法选择30只4w、体重16 ～ 18 g、SPF级Balb/c雌性小鼠,随机分成3组:正常对照组(N)、哮喘组(A)、哮喘+唾液乳杆菌组(AH).应用卵蛋白激发方法建立急性哮喘模型,观察小鼠行为学改变、病理HE染色观察气道炎症改变、电镜观察气管纤毛数量变化,ELISA法测定血清及肺组织匀浆中IgE、IL-4、IFN-γ水平.结果 A组小鼠在4％卵蛋白生理盐水溶液雾化激发5～10 min后出现明显的烦躁不安、头面部瘙痒、抓耳挠腮、呼吸急促、打喷嚏、弓背、前肢缩抬、二便失禁等哮喘急性相表现,AH组小鼠以上反应减轻;病理HE染色见A组支气管管壁增厚、管腔狭窄、气管及血管周围可见大量以嗜酸性粒细胞为主的炎症细胞浸润,管腔中较多炎性分泌物,AH组小鼠肺组织病理改变较哮喘组明显减轻;电镜下,气管纤毛数量A组较N组显著减少,AH组较哮喘组显著增加;血清及肺组织匀浆中A组IgE、IL-4表达水平明显高于N组(P＜0.05),IFN-y表达水平明显低于N组(P＜0.05),而AH组IgE、IL-4表达水平均较A组明显降低,IFN-γ表达水平较A组升高(P＜0.05).结论致敏前唾液乳杆菌灌胃一定程度上减轻了哮喘小鼠的临床症状,部分纠正了Th1/Th2失衡,降低了哮喘模型小鼠的气道炎症.
목적 탐토타액유간균대효천Balb/c소서혈청급폐조직균장중IgE、IL-4급IFN-γ적영향.방법 선택30지4w、체중16 ～ 18 g、SPF급Balb/c자성소서,수궤분성3조:정상대조조(N)、효천조(A)、효천+타액유간균조(AH).응용란단백격발방법건립급성효천모형,관찰소서행위학개변、병리HE염색관찰기도염증개변、전경관찰기관섬모수량변화,ELISA법측정혈청급폐조직균장중IgE、IL-4、IFN-γ수평.결과 A조소서재4％란단백생리염수용액무화격발5～10 min후출현명현적번조불안、두면부소양、조이뇨시、호흡급촉、타분체、궁배、전지축태、이편실금등효천급성상표현,AH조소서이상반응감경;병리HE염색견A조지기관관벽증후、관강협착、기관급혈관주위가견대량이기산성립세포위주적염증세포침윤,관강중교다염성분비물,AH조소서폐조직병리개변교효천조명현감경;전경하,기관섬모수량A조교N조현저감소,AH조교효천조현저증가;혈청급폐조직균장중A조IgE、IL-4표체수평명현고우N조(P＜0.05),IFN-y표체수평명현저우N조(P＜0.05),이AH조IgE、IL-4표체수평균교A조명현강저,IFN-γ표체수평교A조승고(P＜0.05).결론 치민전타액유간균관위일정정도상감경료효천소서적림상증상,부분규정료Th1/Th2실형,강저료효천모형소서적기도염증.
Objective To invstigate the effect of Lactobacillus salivarius on asthma Balb/c mouse in IgE,IL-4 and IFN-γ of serum and lung tissue homogenates.Methods Thirty female Balb/c mice weighing 16-18 g (4 weeks old), SPF grade, were randomly divided into three groups: normal control group (N), asthma group(A), asthma combined Lactobacillus salivarius group (AH).Acute asthma model was established by ovalbumin challenge method, mice were observed in behavioral changes, non-invasive method for the determination of airway responsiveness in mice, the changes in airway inflammation and the number changes of tracheal cilia were observed by pathological HE staining and the electron microscope.Respectively, the levels of IgE and IL-4/IFN-γin the serum and lung tissue homogenate were measured by ELISA.Results five ～ ten min after 4％ OVA saline solution atomization excitation mice in A group appeared obvious restlessness, head and facial itching, scratching, shortness of breath, perioral cyanosis with abdominal breathing, sneezing, stooping, forelimbs shrinkage, urine and feces incontinence and other asthma acute phase performances, The above reaction alleviated in AH group compared to A group;Pathology of HE staining in asthma group showed the thickening bronchial wall, narrow lumen, peribronchial and perivascular infiltration with a large number of eosinophil-based inflammatory cells,lumen with many inflannatory secretions,Pathological changes in lung tissue of mice in AH group significantly alleviated compared with asthma group;Under the electron microscope, the number of cilia in A group significantly decreased compared with N group, while AH group significantly increased compared with A group;Expression level of IgE,IL-4 in the serum and lung homogenate of A group was significantly higher than that of N group (P ＜ 0.05), the level of IFN-γexpression was significantly lower than N group (P ＜ 0.05), however the expression level of IgE, IL-4 in AH group significantly decreased compared with A group (P ＜ 0.05), and IFN-γexpression level elevated compared with A group(P ＜ 0.05).Conclusion Lavage with Lactobacillus salivarius before sensitization could alleviate clinical symptoms of asthmatic mice, partially correct Th1/Th2 imbalance as well as reduce airway infammafion.