CAJ | 학술논문

为了定量评估有机质自组装对其抗微生物矿化作用的影响，从风化褐煤、2种泥炭和1种矿质土壤中分离出的腐植酸用作有机质样品，因为其在来源和有机成分性质方面具有广泛的可变性。以苯-甲醇为提取剂，将原始的腐植酸样品分解成为腐植酸组分和腐植酸-脂质复合物，用碱溶液从腐植酸-脂质复合物中进一步提取出两性腐植酸组分和脂质组分。根据以上各组分在腐植酸中的准确比例，混合各自固体组分，制成与自组装样品化学成分一致的物理混合物。添加原始的腐植酸及其相应的混合物作为唯一的碳源，使用矿质土壤中分离出的微生物菌群开展独立的有氧培养试验。矿化培养125天后，自组装样品矿化量比相应的物理混合物高70％。13C固态核磁共振光谱所得的碳类型分布和疏水性指数结果表明，自组装样品的矿化程度与其化学成分不相关。物理混合物和组分的矿化程度因化学成分而不同，且存在着烷基碳优先矿化的现象。以上研究结果表明，腐植酸的微生物矿化与其自组装程度有关。
위료정량평고유궤질자조장대기항미생물광화작용적영향，종풍화갈매、2충니탄화1충광질토양중분리출적부식산용작유궤질양품，인위기재래원화유궤성분성질방면구유엄범적가변성。이분-갑순위제취제，장원시적부식산양품분해성위부식산조분화부식산-지질복합물，용감용액종부식산-지질복합물중진일보제취출량성부식산조분화지질조분。근거이상각조분재부식산중적준학비례，혼합각자고체조분，제성여자조장양품화학성분일치적물리혼합물。첨가원시적부식산급기상응적혼합물작위유일적탄원，사용광질토양중분리출적미생물균군개전독립적유양배양시험。광화배양125천후，자조장양품광화량비상응적물리혼합물고70％。13C고태핵자공진광보소득적탄류형분포화소수성지수결과표명，자조장양품적광화정도여기화학성분불상관。물리혼합물화조분적광화정도인화학성분이불동，차존재착완기탄우선광화적현상。이상연구결과표명，부식산적미생물광화여기자조장정도유관。
The purpose of this study was to quantitatively assess the effect of organic matter self-assembly on its re-sistance to microbial mineralization. Humic acids isolated from leonardite, two peats and a mineral soil were used as organic matter samples because they provide a broad range of variability in terms of origin and the nature of their or-ganic components. Using a benzene-methanol extraction the original humic acid samples were disassembled into humic components and a humic-lipid composite. The composite was further disassembled by using an alkaline aqueous extrac-tion into humic amphiphilic and lipid components. Mixtures that reproduced the composition of self-assembled samples were prepared by mixing the solid individual fractions in the exact proportions that they were present in the original ma-terial. The original humic acid and their corresponding mixtures were added as the sole carbon source in separate aero-bic cultures containing a microbial consortium isolated from a mineral soil. After incubation for 125 days mineralization of the self-assembled samples was shown to be higher by as much as 70% compared to their corresponding physical mixtures. The extent of mineralization of the selfassembled samples was not correlated to chemical composition base on the carbon-type distribution or hydrophobicity index derived from13C solid-state NMR spectra. Mineralization of the physical mixtures and fractions did vary with chemical composition and was accompanied by preferential mineraliza-tion of alkyl carbon. These results suggest the microbial mineralization of humic acids is related to their self-assembly.