CAJ | 학술논문

研究猫须草多酚的提取工艺及其抗氧化活性。通过单因素试验探讨提取剂、乙醇体积分数、料液比、提取温度、提取时间、提取次数等因素对猫须草多酚提取率的影响,在单因素试验的基础上进行正交试验,结果表明,猫须草多酚的最佳提取工艺条件如下：10%乙醇,料液比1∶30,提取温度90℃,提取时间40min,在该条件下猫须草多酚的提取率为11．99mg·g-1。通过测定猫须草多酚清除DPPH自由基、超氧阴离子自由基、亚硝酸根离子的能力及其还原力,对猫须草多酚的抗氧化活性进行评价,结果表明,在一定质量浓度范围内,猫须草多酚清除DPPH自由基的能力及还原力优于Vc,清除超氧阴离子自由基的能力与Vc接近,但清除亚硝酸根离子的能力低于Vc。
연구묘수초다분적제취공예급기항양화활성。통과단인소시험탐토제취제、을순체적분수、료액비、제취온도、제취시간、제취차수등인소대묘수초다분제취솔적영향,재단인소시험적기출상진행정교시험,결과표명,묘수초다분적최가제취공예조건여하：10%을순,료액비1∶30,제취온도90℃,제취시간40min,재해조건하묘수초다분적제취솔위11．99mg·g-1。통과측정묘수초다분청제DPPH자유기、초양음리자자유기、아초산근리자적능력급기환원력,대묘수초다분적항양화활성진행평개,결과표명,재일정질량농도범위내,묘수초다분청제DPPH자유기적능력급환원력우우Vc,청제초양음리자자유기적능력여Vc접근,단청제아초산근리자적능력저우Vc。
Extraction process and antioxidant activity of polyphenols from Clerodendranthus spicatus were studied. The affect factors such as extractant,ethanol content,solid-liquid ratio,extraction time,temperature and extraction times were investigated by single factor test. Orthogonal array method was carried out on the basis of single factor experiment,10%ethanol,solid-liquid ratio of 1∶30, extraction temperature of 90℃ and time of 40min were found optimal and maximum extraction rate of polyphenols was 11. 99 mg· g-1 . Reducing power and ability to scavenge DPPH·,O2-·and NO2-were determined respectively and antioxidant activity of poly-phenols from Clerodendranthus spicatus were evaluated. Compared with Vc,DPPH·scavenging activity and reducing power of the polyphenols were fairly high,but nitrite scavenging activity was relatively poor,and O2-scavenging activity was similar to Vc with-ithin the experimental range.