医药前沿
醫藥前沿
의약전연
Yiayao Qianyan
2015年
26期
372-373
,共2页
消食健胃片%绿原酸%高效液相色谱
消食健胃片%綠原痠%高效液相色譜
소식건위편%록원산%고효액상색보
Chlorogenic acid%Xiao Shi Jian Wei Pian%HPLC
目的:建立消食健胃片中绿原酸含量测定的方法.方法:采用高效液相色谱法,以ZORBAX SB-C18(4.6mm×150mm,5μ)为色谱柱,流动相为乙腈-0.4%磷酸(13:87),流速1.0 mL?min-1,检测波长为327nm.结果 绿原酸在0.0050mg?mL-1~0.0500 m g?m L-1范围内线性关系良好(r=1.0000),平均回收率为99.59%, R S D=0.94%.结论:该方法准确、可靠、重复性好、专一性强,可用于消食健胃片中绿原酸的含量测定.
目的:建立消食健胃片中綠原痠含量測定的方法.方法:採用高效液相色譜法,以ZORBAX SB-C18(4.6mm×150mm,5μ)為色譜柱,流動相為乙腈-0.4%燐痠(13:87),流速1.0 mL?min-1,檢測波長為327nm.結果 綠原痠在0.0050mg?mL-1~0.0500 m g?m L-1範圍內線性關繫良好(r=1.0000),平均迴收率為99.59%, R S D=0.94%.結論:該方法準確、可靠、重複性好、專一性彊,可用于消食健胃片中綠原痠的含量測定.
목적:건립소식건위편중록원산함량측정적방법.방법:채용고효액상색보법,이ZORBAX SB-C18(4.6mm×150mm,5μ)위색보주,류동상위을정-0.4%린산(13:87),류속1.0 mL?min-1,검측파장위327nm.결과 록원산재0.0050mg?mL-1~0.0500 m g?m L-1범위내선성관계량호(r=1.0000),평균회수솔위99.59%, R S D=0.94%.결론:해방법준학、가고、중복성호、전일성강,가용우소식건위편중록원산적함량측정.
Objective To study and establish the reversed phased high performance liquid chromatography method for determination of Chlorogenic acid in Xiao Shi Jian Wei Pian.Method The chromatographic conditions were as follows: Agilent ZORBAX SB-C18(4.6×150mm, 5μ),column was used as stationary phase,the mobile phase was composed of Acetonitrile and 0.4% phosphoric acid solution (13:87), the flow rate was 1.0 mL?min-1;the UV absorbance detection wavelength was set at 327nm.Results The Chlorogenic acid curve was linear within the range of 0.0050-0.0500 mg?mL-1(r=1.0000);The average recovery of Chlorogenic acid was 99.59%.Conclusion The result showed that this method was convenient、rapid、sensitive、reproducible and accurate. It can be used in determination of Chlorogenic acid in Xiao Shi Jian Wei Pian.
