CAJ | 학술논문

目的：研究胃癌组织及其邻近胃组织中睾丸特异性Y样蛋白5( testis-specific Y-like pro-tein 5,TSPYL5)的表达与预后及多个临床病理变量之间的关系,同时分析其是否在胃癌远期生存的危险因素中具有意义。方法采用RT-PCR方法检测44例胃癌组织、癌旁胃正常黏膜组织中TSPYL5 mRNA的表达,分析其表达与胃癌相关临床病理变量的关系。结果与癌旁组织相比,胃癌组织中TSPYL5表达明显下调的有24例,占总数的54.5%。 TSPYL5的表达与TNM分期、淋巴转移及是否远处转移有关,而与肿瘤的浸润深度、大小及患者性别、年龄等无关(P<0.05)。肿瘤组织与癌旁组织的TSPYL5表达量相比,其表达下调者的DFS远低于无明显变化及上调者(P=0.01)。经单因素和多因素COX分析表明,TSPYL5可作为胃癌的独立预测因素(P<0.001)。结论 TSPYL5作为抑癌基因,其表达缺失参与了胃癌的发生发展,同时TSPYL5可作为判断胃癌预后的重要分子标志。
목적：연구위암조직급기린근위조직중고환특이성Y양단백5( testis-specific Y-like pro-tein 5,TSPYL5)적표체여예후급다개림상병리변량지간적관계,동시분석기시부재위암원기생존적위험인소중구유의의。방법채용RT-PCR방법검측44례위암조직、암방위정상점막조직중TSPYL5 mRNA적표체,분석기표체여위암상관림상병리변량적관계。결과여암방조직상비,위암조직중TSPYL5표체명현하조적유24례,점총수적54.5%。 TSPYL5적표체여TNM분기、림파전이급시부원처전이유관,이여종류적침윤심도、대소급환자성별、년령등무관(P<0.05)。종류조직여암방조직적TSPYL5표체량상비,기표체하조자적DFS원저우무명현변화급상조자(P=0.01)。경단인소화다인소COX분석표명,TSPYL5가작위위암적독립예측인소(P<0.001)。결론 TSPYL5작위억암기인,기표체결실삼여료위암적발생발전,동시TSPYL5가작위판단위암예후적중요분자표지。
Objective To study the relationship between the expression of TSPYL5 ( testis-specific Y-like protein 5,TSPYL5)in gastric carcinoma and adjacent gastric tissues and the long term survival. Our study is to investigate the clinical significances of expression of TSPYL5 in gastric cancer. Methods The mRNA level of TSPYL5 in 44 matched samples comprising primary gastric carcinoma and paired non-cancerous mucosa were detected and compared by quantitative RT-PCR. Then the correlations of mRNA level of TSPYL5 with the clini-copathological characteristics and prognosis of patients with gastric cancer were analyzed. Results The rate of TSPYL5 mRNA in the primary gastric cancer tissues was significantly higher than that in the adjacent non-can-cerous tissues(54. 5%). mRNA expression of TSPYL5 was associated with certain clinical-pathologic variables such as advancing TNM stage, lymphatic metastasis, distant metastasis. We obtained the expression levels ratio of cancer tissue and adjacent non-cancerous tissues and found the lower ratio with a lower Disease-Free Survival (DFS)(P<0. 001). TSPYL5,as a tumor suppressor gene, remained a statistically-significant prognostic marker in the COX regression analysis. Conclusion Down-expression of TSPYL5 had a significant effect on develop-ment and progress in gastric cancer,which can be used to prognose gastric carcinoma.