检验医学与临床
檢驗醫學與臨床
검험의학여림상
Laboratory Medicine and Clinic
2015年
21期
3184-3185
,共2页
Genefinder%样品预染法染色%胶内染色
Genefinder%樣品預染法染色%膠內染色
Genefinder%양품예염법염색%효내염색
Genefinder%staining with preparative dye liquor%staining in agarose gel electrophoresis
目的：确定琼脂糖凝胶电泳中使用核酸染料Genefinder的最适方法，以期找到染色效果理想的染色方法。方法在琼脂糖凝胶电泳中，采用预染样品法、胶内染色法以及两者相结合的3种方法，使用核酸染料Genefinder进行染色，对染色结果进行比较分析。结果使用胶内染色法染色效果较好，预染样品法次之，胶染及样品染色法结合效果最差。结论核酸染料Genefinder会影响DNA的迁移效率，使用Genefinder进行胶内染色是一种较好的染色方法。
目的：確定瓊脂糖凝膠電泳中使用覈痠染料Genefinder的最適方法，以期找到染色效果理想的染色方法。方法在瓊脂糖凝膠電泳中，採用預染樣品法、膠內染色法以及兩者相結閤的3種方法，使用覈痠染料Genefinder進行染色，對染色結果進行比較分析。結果使用膠內染色法染色效果較好，預染樣品法次之，膠染及樣品染色法結閤效果最差。結論覈痠染料Genefinder會影響DNA的遷移效率，使用Genefinder進行膠內染色是一種較好的染色方法。
목적：학정경지당응효전영중사용핵산염료Genefinder적최괄방법，이기조도염색효과이상적염색방법。방법재경지당응효전영중，채용예염양품법、효내염색법이급량자상결합적3충방법，사용핵산염료Genefinder진행염색，대염색결과진행비교분석。결과사용효내염색법염색효과교호，예염양품법차지，효염급양품염색법결합효과최차。결론핵산염료Genefinder회영향DNA적천이효솔，사용Genefinder진행효내염색시일충교호적염색방법。
Objective To determine a optimal method for using nucleic acid dyes Genefinder in agarose gel e‐lectrophoresis in order to find out a staining method with ideal dyeing effect .Methods The pre‐dyeing sample meth‐od ,gel staining method and their combination staining method were adopted to perform the staining with nucleic acid dyes in agarose gel electrophoresis .The staining results were performed the comparative analysis .Results The Genefinder staining had better effect ,the pre‐dyeing sample method took the second place and the combination stai‐ning method had the worst effect .Conclusion Nucleic acid dyes Genefinder has an effect on the mobility of nucleic acid in agarose gel electrophoresis ,therefore staining nucleic acid with Genefinder is a better staining method in agar‐ose gel electrophoresis .
