检验医学与临床
檢驗醫學與臨床
검험의학여림상
Laboratory Medicine and Clinic
2015年
21期
3182-3183,3185
,共3页
生殖器疱疹%病毒培养%荧光定量聚合酶链反应%酶联免疫吸附试验
生殖器皰疹%病毒培養%熒光定量聚閤酶鏈反應%酶聯免疫吸附試驗
생식기포진%병독배양%형광정량취합매련반응%매련면역흡부시험
genital herpes%virus culture%FQ-PCR%ELISA
目的：寻找更为准确、经济、实用的生殖器疱疹病毒感染的检测方法。方法取根据病史及典型的临床症状表现的生殖器疱疹疑似病例患者标本50例，分别采用病毒培养、荧光定量聚合酶链反应（FQ‐PCR）及酶联免疫吸附试验（ELISA）3种方法进行实验室检测，比较各种方法检测的阳性率。结果病毒培养阳性30例，阳性率60．0％；FQ‐PCR检测阳性34例，阳性率68．0％；ELISA检测阳性32例，阳性率64．0％，经χ2检验，三者差异无统计学意义（χ2＝0．69，P＞0．05）。水疱和脓疱标本的阳性率分别为94．6％和83．3％，明显高于其他来源标本的阳性率。结论3种检测方法对临床疑似生殖器疱疹患者的检测阳性率差异无统计学意义（P＞0．05）。相对另外两种检测方法，ELISA因其便宜、实用的优点而适用于临床推广应用。水疱和脓疱标本阳性率明显高于其他类型标本的阳性率，对临床疑似病例的实验诊断，标本以采集水疱和脓疱液为佳。
目的：尋找更為準確、經濟、實用的生殖器皰疹病毒感染的檢測方法。方法取根據病史及典型的臨床癥狀錶現的生殖器皰疹疑似病例患者標本50例，分彆採用病毒培養、熒光定量聚閤酶鏈反應（FQ‐PCR）及酶聯免疫吸附試驗（ELISA）3種方法進行實驗室檢測，比較各種方法檢測的暘性率。結果病毒培養暘性30例，暘性率60．0％；FQ‐PCR檢測暘性34例，暘性率68．0％；ELISA檢測暘性32例，暘性率64．0％，經χ2檢驗，三者差異無統計學意義（χ2＝0．69，P＞0．05）。水皰和膿皰標本的暘性率分彆為94．6％和83．3％，明顯高于其他來源標本的暘性率。結論3種檢測方法對臨床疑似生殖器皰疹患者的檢測暘性率差異無統計學意義（P＞0．05）。相對另外兩種檢測方法，ELISA因其便宜、實用的優點而適用于臨床推廣應用。水皰和膿皰標本暘性率明顯高于其他類型標本的暘性率，對臨床疑似病例的實驗診斷，標本以採集水皰和膿皰液為佳。
목적：심조경위준학、경제、실용적생식기포진병독감염적검측방법。방법취근거병사급전형적림상증상표현적생식기포진의사병례환자표본50례，분별채용병독배양、형광정량취합매련반응（FQ‐PCR）급매련면역흡부시험（ELISA）3충방법진행실험실검측，비교각충방법검측적양성솔。결과병독배양양성30례，양성솔60．0％；FQ‐PCR검측양성34례，양성솔68．0％；ELISA검측양성32례，양성솔64．0％，경χ2검험，삼자차이무통계학의의（χ2＝0．69，P＞0．05）。수포화농포표본적양성솔분별위94．6％화83．3％，명현고우기타래원표본적양성솔。결론3충검측방법대림상의사생식기포진환자적검측양성솔차이무통계학의의（P＞0．05）。상대령외량충검측방법，ELISA인기편의、실용적우점이괄용우림상추엄응용。수포화농포표본양성솔명현고우기타류형표본적양성솔，대림상의사병례적실험진단，표본이채집수포화농포액위가。
Objective To search for a more accurate ,economic and practical detection method of genital herpes virus (HSV) .Methods 50 specimens were collected from the suspected as genital herpes according to the disease history and the typical clinical symptoms and tested by adopting 3 kinds of method :virus culture method ,fluorescence quantitative‐PCR (FQ‐PCR) and ELISA .The positive rates were compared among 3 kinds of method .Results 30 ca‐ses were positive in the virus culture ,the positive rate was 60 .0% ,34 cases were positive in FQ‐PCR with the posi‐tive rate of 68 .0% ,32 cases were positive in ELISA with the positive rate of 64 .0% ,the differences were not statisti‐cally significant(χ2 =0 .69 ,P>0 .05) .However ,the positive rates of blister and pustules specimens were 94 .6% and 83 .3% respectively ,which were significantly higher than those of other sources of samples .Conclusion The positive rate of clinically suspected genital herpes detected by using three methods had no statistically significant difference . ELISA is more suitable for clinical application compared with other two methods because of cheap and convenient ad‐vantages .The positive rate of blister and pustules specimens is significantly higher than that of other types of sam‐ples ,therefore in the laboratory diagnosis for the suspected case ,collecting the blister and pustules samples is best .
