药品评价
藥品評價
약품평개
Drug Evaluation
2015年
20期
22-25
,共4页
组织块法%胶原酶两步灌流法%大鼠原代肝细胞%产量%活率%活力
組織塊法%膠原酶兩步灌流法%大鼠原代肝細胞%產量%活率%活力
조직괴법%효원매량보관류법%대서원대간세포%산량%활솔%활력
Tissue Block Method%Two-step Collagenase Perfusion Method%Output%Rats Hepatocytes%Living rate%Vitality
目的：比较组织块法和胶原酶两步灌流法制备大鼠原代肝细胞的产量、活率和活力。方法：通过荧光倒置显微镜观察大鼠原代肝细胞的形态，并且分别通过台盼蓝染色和MTT法测定制备得到的原代肝细胞产量、活率和活力。结果：组织块法和胶原酶两步灌流法制备大鼠原代肝细胞的产量分别为(0.30±0.10)×108个/肝和(0.67±0.11)×108个/肝，活率分别为(65±14)%和(88±16)%，组织块法制备大鼠原代肝细胞的活力比胶原酶两步灌流法更低，且活力下降快。结论：胶原酶两步灌流法制备的大鼠原代肝细胞比组织块法产量更高、活率和活力均更好，更适用于体外药物代谢的研究。
目的：比較組織塊法和膠原酶兩步灌流法製備大鼠原代肝細胞的產量、活率和活力。方法：通過熒光倒置顯微鏡觀察大鼠原代肝細胞的形態，併且分彆通過檯盼藍染色和MTT法測定製備得到的原代肝細胞產量、活率和活力。結果：組織塊法和膠原酶兩步灌流法製備大鼠原代肝細胞的產量分彆為(0.30±0.10)×108箇/肝和(0.67±0.11)×108箇/肝，活率分彆為(65±14)%和(88±16)%，組織塊法製備大鼠原代肝細胞的活力比膠原酶兩步灌流法更低，且活力下降快。結論：膠原酶兩步灌流法製備的大鼠原代肝細胞比組織塊法產量更高、活率和活力均更好，更適用于體外藥物代謝的研究。
목적：비교조직괴법화효원매량보관류법제비대서원대간세포적산량、활솔화활력。방법：통과형광도치현미경관찰대서원대간세포적형태，병차분별통과태반람염색화MTT법측정제비득도적원대간세포산량、활솔화활력。결과：조직괴법화효원매량보관류법제비대서원대간세포적산량분별위(0.30±0.10)×108개/간화(0.67±0.11)×108개/간，활솔분별위(65±14)%화(88±16)%，조직괴법제비대서원대간세포적활력비효원매량보관류법경저，차활력하강쾌。결론：효원매량보관류법제비적대서원대간세포비조직괴법산량경고、활솔화활력균경호，경괄용우체외약물대사적연구。
Object: To compare the different of output, living rate and vitality between tissue block method and two steps collagenase perfusion method for preparation ratshepatocytes.Method:Observe the shape of rat hepatocytes by fluorescent inverted microscope, and determine the output, living rate and vitality of rats hepatocytes by Trypanblue stainand MTT assay respectively.Results:The output of rats hepatocytes prepared by tissue block method and two steps collagenase perfusion was (0.30±0.10)x108 per liver and (0.67±0.11)x108 per liver, respectively. The living rate was (65±14)% and (88±16)%, respectively. The vitality of rats hepatocytes prepared by tissue block method was lower than that by two-step collagenase perfusion method and the vitality down faster. Conclusion:The output of rats hepatocytes prepared by two-step collagenase perfusion method was higher than that by tissue block method, as well as living rate and vitality. Two-step collagenase perfusion method is more suitable for the in vitro drug metabolism study.
