中国动物检疫
中國動物檢疫
중국동물검역
Chinese Journal of Animal Health Inspection
2015年
10期
28-31
,共4页
李明勇%宋大伟%王志全
李明勇%宋大偉%王誌全
리명용%송대위%왕지전
兔源%大肠杆菌%超广谱β-内酰胺酶(ESBL)%SoxS基因%多重耐药性
兔源%大腸桿菌%超廣譜β-內酰胺酶(ESBL)%SoxS基因%多重耐藥性
토원%대장간균%초엄보β-내선알매(ESBL)%SoxS기인%다중내약성
rabbit%Escherichia coli%extended-spectrumβ-lactamase(ESBL)%SoxS gene%multiple antibiotic resistance
[目的]了解兔源产ESBL大肠杆菌耐药特点，为掌握其造成的耐药性危害提供数据参考。[方法]2015年3-4月从青岛4个养兔场采集118份兔粪便棉拭子，进行大肠杆菌分离，并检测对16种抗生素的耐药谱，及对多重耐药调控基因SoxS携带率。[结果]从118份兔粪便棉拭子分离获得68株大肠杆菌，确证筛选出47株产ESBL大肠杆菌，占兔源大肠杆菌的69.1%。药敏结果显示所有产ESBL大肠杆菌菌株耐药性较强且呈现多重耐药，仅对碳青霉烯类药物敏感率100%。兔源产ESBL大肠杆菌多重耐药调控基SoxS携带率为100%，且不同动物源性产ESBL大肠杆菌的多重耐药调控基因同源关系较近。[结论]青岛地区兔源产ESBL大肠杆菌分离率高且耐药谱广，多重耐药调控基因SoxS携带率高，主要涉及到细菌主动外排泵出机制，使得大肠杆菌对抗生素的敏感性中起到了至关重要的作用，本研究对抗生素在临床中的应用起指导意义。
[目的]瞭解兔源產ESBL大腸桿菌耐藥特點，為掌握其造成的耐藥性危害提供數據參攷。[方法]2015年3-4月從青島4箇養兔場採集118份兔糞便棉拭子，進行大腸桿菌分離，併檢測對16種抗生素的耐藥譜，及對多重耐藥調控基因SoxS攜帶率。[結果]從118份兔糞便棉拭子分離穫得68株大腸桿菌，確證篩選齣47株產ESBL大腸桿菌，佔兔源大腸桿菌的69.1%。藥敏結果顯示所有產ESBL大腸桿菌菌株耐藥性較彊且呈現多重耐藥，僅對碳青黴烯類藥物敏感率100%。兔源產ESBL大腸桿菌多重耐藥調控基SoxS攜帶率為100%，且不同動物源性產ESBL大腸桿菌的多重耐藥調控基因同源關繫較近。[結論]青島地區兔源產ESBL大腸桿菌分離率高且耐藥譜廣，多重耐藥調控基因SoxS攜帶率高，主要涉及到細菌主動外排泵齣機製，使得大腸桿菌對抗生素的敏感性中起到瞭至關重要的作用，本研究對抗生素在臨床中的應用起指導意義。
[목적]료해토원산ESBL대장간균내약특점，위장악기조성적내약성위해제공수거삼고。[방법]2015년3-4월종청도4개양토장채집118빈토분편면식자，진행대장간균분리，병검측대16충항생소적내약보，급대다중내약조공기인SoxS휴대솔。[결과]종118빈토분편면식자분리획득68주대장간균，학증사선출47주산ESBL대장간균，점토원대장간균적69.1%。약민결과현시소유산ESBL대장간균균주내약성교강차정현다중내약，부대탄청매희류약물민감솔100%。토원산ESBL대장간균다중내약조공기SoxS휴대솔위100%，차불동동물원성산ESBL대장간균적다중내약조공기인동원관계교근。[결론]청도지구토원산ESBL대장간균분리솔고차내약보엄，다중내약조공기인SoxS휴대솔고，주요섭급도세균주동외배빙출궤제，사득대장간균대항생소적민감성중기도료지관중요적작용，본연구대항생소재림상중적응용기지도의의。
Objective] To understand the antimicrobial characteristics of ESBL-producingE. colistrains from rabbits and provide important data for reference to know their antimicrobial hazard. [Methods] 118 rabbit fecal samples were taken from 4 rabbit farms in Qingdao region forE. coli isolation and anti-microbial identification. [Results] 68E. coli strains were isolated from the 118 rabbit fecal samples with 47 ESBL-producingE. coli strains,all of which were multi-drug resistant but sensitive to carbapenems. Polymerase chain reaction and cloning of the SoxS gene demonstrated that the multi-drug resistant SoxS gene carrying rate of ESBL-producingE. coli strains from rabbit was 100%. [Conclusion] The isolation rate of ESBL-producingE. colistrains from Qingdao region was high with wide drug resistance spectrum. High rate of multi-drug resistant SoxS gene was mainly involved in the bacterial efflux pump mechanism,playing key role in sensitivity ofE. coli to antibiotics. The study provided guidance for clinical application of antibiotics.
