CAJ | 학술논문

目的：探讨高脂血、溶血标本对荧光定量聚合酶链反应（PCR）测定低水平 HBV‐DNA检测结果的影响。方法对收治的100例三酰甘油（TG）正常（TG≤1．46 mmol／L）、实时荧光定量PCR测定为（4．0～9．0）×103 IU／mL的乙型肝炎患者采集血清标本，进行即刻检测，另外选择100份乙肝五项全阴且测不出HBV‐DNA的高TG标本和100份乙肝五项全阴且测不出 HBV‐DNA的全血标本分别制备成高脂血、溶血血清标本，同时进行高脂血和非高脂血、溶血血清和非溶血血清的 HBV‐DNA荧光定量PCR检测，并进行配对 t检验。结果高脂血和非高脂血、溶血血清和非溶血血清的 HBV‐DNA水平差异有统计学意义（P＜0．05）。结论高脂血、溶血标本低水平HBV‐DNA荧光定量PCR定量检测结果较空腹采集即刻检测的结果降低。
목적：탐토고지혈、용혈표본대형광정량취합매련반응（PCR）측정저수평 HBV‐DNA검측결과적영향。방법대수치적100례삼선감유（TG）정상（TG≤1．46 mmol／L）、실시형광정량PCR측정위（4．0～9．0）×103 IU／mL적을형간염환자채집혈청표본，진행즉각검측，령외선택100빈을간오항전음차측불출HBV‐DNA적고TG표본화100빈을간오항전음차측불출 HBV‐DNA적전혈표본분별제비성고지혈、용혈혈청표본，동시진행고지혈화비고지혈、용혈혈청화비용혈혈청적 HBV‐DNA형광정량PCR검측，병진행배대 t검험。결과고지혈화비고지혈、용혈혈청화비용혈혈청적 HBV‐DNA수평차이유통계학의의（P＜0．05）。결론고지혈、용혈표본저수평HBV‐DNA형광정량PCR정량검측결과교공복채집즉각검측적결과강저。
Objective To investigate the influence of heperlipemia and hemolysis specimens on the fluorescence quantitative PCR determination results of low level HBV‐DNA .Methods The serum samples were collected from 100 hepatitis B patients with normal triacylglycerol (TG) (≤1 .46 mmol/L and (4 .0-9 .0) × 103 IU/mL by real time fluorescence quantitative PCR) in our hospital and performed the instant detection .In addition ,100 high TG samples of hepatitis B 5‐item negative and HBV‐DNA non‐detection and 100 whole blood samples of hepatitis B 5‐item nega‐tive and HBV‐DNA non‐detection were collected and performed the simultaneous HBV‐DNA detection of fluores‐cence quantitative PCR on the hyperlipemia and non‐hyperlipemia specimens ,hemolysis specimen and non‐hemolytic serum .And the paired t test was conducted .Results The HBV‐DNA detection levels had statistically significant differences between hyperlipemia and non‐hyperlipemia specimens and between hemolytic serum and non‐hemolytic serum (P<0 .05) .Conclusion The fluorescence quantitative PCR detection results of hyperlipemia and hemolysis specimens of low HBV‐DNA level are reduced compared with those of instant detection for fasting collection speci‐mens .