世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
World Science and Technology-Modernization of Traditional Chinese Medicine
2015年
9期
1785-1789
,共5页
张雪%赵炳祥%宋玉琴%杨雨婷%董艳红%谢晓芳%彭成
張雪%趙炳祥%宋玉琴%楊雨婷%董豔紅%謝曉芳%彭成
장설%조병상%송옥금%양우정%동염홍%사효방%팽성
人参皂苷Rb1%乌头碱%心肌细胞%能量代谢
人參皂苷Rb1%烏頭堿%心肌細胞%能量代謝
인삼조감Rb1%오두감%심기세포%능량대사
Ginsenosides Rb1%aconitine%cardiomyocytes%energy metabolism
目的:探讨乌头碱配伍人参皂苷Rb1对心肌细胞能量代谢的影响。方法:采用胰蛋白酶消化和差速贴壁法培养大鼠乳鼠心肌细胞,分为正常对照组、0.2%乌头碱组、0.1%乌头碱组、人参皂苷Rb1组、乌头碱配伍人参皂苷Rb1组(1?1、1?2、2?1),给药作用1 h,检测心肌细胞的细胞活力和体内糖原、琥珀酸脱氢酶、细胞色素C氧化酶以及细胞乳酸脱氢酶的含量。结果:0.2%乌头碱能够明显降低心肌细胞活力,增加乳酸脱氢酶含量,降低细胞内糖原、琥珀酸脱氢酶、细胞色素C氧化酶含量;1×10-6 mol·L-1或2×10-6 mol·L-1浓度的人参皂苷Rb1配伍乌头碱,可明显对抗0.2%乌头碱对心肌细胞的毒性。结论:人参皂苷Rb1可降低乌头碱心肌细胞毒性,其作用机制可能与对抗细胞能量代谢有关。
目的:探討烏頭堿配伍人參皂苷Rb1對心肌細胞能量代謝的影響。方法:採用胰蛋白酶消化和差速貼壁法培養大鼠乳鼠心肌細胞,分為正常對照組、0.2%烏頭堿組、0.1%烏頭堿組、人參皂苷Rb1組、烏頭堿配伍人參皂苷Rb1組(1?1、1?2、2?1),給藥作用1 h,檢測心肌細胞的細胞活力和體內糖原、琥珀痠脫氫酶、細胞色素C氧化酶以及細胞乳痠脫氫酶的含量。結果:0.2%烏頭堿能夠明顯降低心肌細胞活力,增加乳痠脫氫酶含量,降低細胞內糖原、琥珀痠脫氫酶、細胞色素C氧化酶含量;1×10-6 mol·L-1或2×10-6 mol·L-1濃度的人參皂苷Rb1配伍烏頭堿,可明顯對抗0.2%烏頭堿對心肌細胞的毒性。結論:人參皂苷Rb1可降低烏頭堿心肌細胞毒性,其作用機製可能與對抗細胞能量代謝有關。
목적:탐토오두감배오인삼조감Rb1대심기세포능량대사적영향。방법:채용이단백매소화화차속첩벽법배양대서유서심기세포,분위정상대조조、0.2%오두감조、0.1%오두감조、인삼조감Rb1조、오두감배오인삼조감Rb1조(1?1、1?2、2?1),급약작용1 h,검측심기세포적세포활력화체내당원、호박산탈경매、세포색소C양화매이급세포유산탈경매적함량。결과:0.2%오두감능구명현강저심기세포활력,증가유산탈경매함량,강저세포내당원、호박산탈경매、세포색소C양화매함량;1×10-6 mol·L-1혹2×10-6 mol·L-1농도적인삼조감Rb1배오오두감,가명현대항0.2%오두감대심기세포적독성。결론:인삼조감Rb1가강저오두감심기세포독성,기작용궤제가능여대항세포능량대사유관。
This article was aimed to discuss the impact of compatibility effect of aconitine and ginsenosides Rb1 on energy metabolism of cardiomyocytes. The trypsin was used to digest and differential adherence method was used to purify and cultivate cardiomyocytes. Rat cardiomyocytes were divided into the normal control group, 0.2% aconitine group, 0.1% aconitine group, ginsenosides Rb1group, compatibility of aconitine and ginsenosides Rb1 (1?1, 1?2, 2?1) group. The drug reaction time was 1 hour. The cell vitality, glycogen content, succinate dehydrogenase, cytochrome C oxidase and LDH concentration were detected in cardiomyocytes. The results showed that 0.2% aconitine significantly reduced myocardial viability, increased the LDH concentration, reduced the glycogen content, succinate dehydrogenase and cytochrome C oxidase. The compatibility of 1×10-6 mol·L-1 or 2×10-6 mol·L-1 ginsenosides Rb1 and aconitine was significantly against the cardiomyocytes toxicity induced by 0.2% aconitine. It was concluded that ginsenosides Rb1 can reduce the cardiomyocytes toxicity by aconitine. Its mechanism may be associated with the cellular energy metabolism.