CAJ | 학술논문

本研究针对鲤科疱疹病毒2型（CyHV-2）的主要衣壳蛋白基因，设计了特异性引物和Taqman探针，建立了实时荧光PCR方法。其检测下限为83拷贝，标准曲线R2＞0.998，且经比对，灵敏度与现行方法相当。选取了健康异育银鲫、金鱼、鲤鱼以及常见4种水生DNA病毒以及7种水生环境细菌进行特异性实验，结果均无交叉反应。重复性实验显示，2份独立的15次重复结果变异系数均小于2%，说明本方法重复性好。本方法能作为目前CyHV-2疫病检测方法的有效补充，对该病的防控具有重要的意义。
본연구침대리과포진병독2형（CyHV-2）적주요의각단백기인，설계료특이성인물화Taqman탐침，건립료실시형광PCR방법。기검측하한위83고패，표준곡선R2＞0.998，차경비대，령민도여현행방법상당。선취료건강이육은즉、금어、리어이급상견4충수생DNA병독이급7충수생배경세균진행특이성실험，결과균무교차반응。중복성실험현시，2빈독립적15차중복결과변이계수균소우2%，설명본방법중복성호。본방법능작위목전CyHV-2역병검측방법적유효보충，대해병적방공구유중요적의의。
Primers and Taqman probe targeting major capsid protein of cyprinid herpesvirus 2(CyHV-2)were designed and a real-time PCR method was established. The lower limit of detection was 83 copies,and standard curve R2>0.998. Comparison experiments showed that the sensitivity of this method was of same level as existing method.Healthy Carassius auratus gibelio,Carassius auratus auratus,Cyprinus Carpio,4 aquatic viruses and 7 aquatic bacteria were included for specificity tests,no cross reaction was observed. Repeatability tests showed that both coefficients of varia-tion of independent result repeated 15 times were smaller than 2%,suggesting that the repeatability of this method was ideal. The established method would be supplementary to the existing CyHV-2 detection methods,and was of important significance for disease control.