CAJ | 학술논문

目的：文章通过复合酶法辅助提取刺五加叶中的金丝桃苷，并利用正交试验优化其提取工艺。方法：利用高效液相色谱法，以金丝桃苷为检测指标，对加酶量、温度、时间和pH值等因素，采用正交试验及方差分析法，以确定金丝桃苷复合酶法最佳提取工艺。结果：单因素试验的结果表明，各种酶对金丝桃苷提取率均有一定程度的提高，各因素的影响水平从高到低分别为pH、中性蛋白酶、温度、时间、果胶酶、木聚糖酶和纤维素酶。通过正交试验分析可得：在复合酶为2%果胶酶、2%木聚糖酶、0.5%中性蛋白酶和0.5%纤维素酶，提取温度为30℃，提取为时间为10 min，pH=4.5时，提取效果最佳，提取率为1.84%，与传统工艺相比，金丝桃苷产率提高了107%。结论：使用复合酶法辅助提取金丝桃苷可较大的提高产率，具有一定经济价值。
목적：문장통과복합매법보조제취자오가협중적금사도감，병이용정교시험우화기제취공예。방법：이용고효액상색보법，이금사도감위검측지표，대가매량、온도、시간화pH치등인소，채용정교시험급방차분석법，이학정금사도감복합매법최가제취공예。결과：단인소시험적결과표명，각충매대금사도감제취솔균유일정정도적제고，각인소적영향수평종고도저분별위pH、중성단백매、온도、시간、과효매、목취당매화섬유소매。통과정교시험분석가득：재복합매위2%과효매、2%목취당매、0.5%중성단백매화0.5%섬유소매，제취온도위30℃，제취위시간위10 min，pH=4.5시，제취효과최가，제취솔위1.84%，여전통공예상비，금사도감산솔제고료107%。결론：사용복합매법보조제취금사도감가교대적제고산솔，구유일정경제개치。
This study was aimed to optimize the extraction process of hyperoside from leaves ofAcanthopanax senticosusby compounding-enzyme method and orthogonal experiment. The hyperoside compound was regarded as standard and determined by HPLC. Based on the experiments of 4 factors including the enzyme amount, temperature, extraction time and PH values, the extraction process of hyperoside was determined by the orthogonal experiments and variance analysis. The results of single-factor experiments showed that different enzymes showed different effects on the enhance yield of hyperoside. The effects of different factors showed that the order of PH, neutral protease, temperature, time, pectinase, xylanase and cellulose was from strong to weak. Through orthogonal analysis, the optimum conditions were 2% pectinase, 2% xylanase, 0.5% neutral protease, and 0.5% cellulose, under the temperature of 30°C, extraction time of 10 min, and pH = 4.5. Under these conditions, the extraction rate was 1.84%. The yield was increased 107% compared with traditional process. It was concluded that the use of compounding enzyme can increase the yield of hyperoside, which possessed a lot of economic benefits.