世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
World Science and Technology-Modernization of Traditional Chinese Medicine
2015年
9期
1813-1817
,共5页
李锐华%徐小倩%张小强%李英%吴云%丁岗%王振中%萧伟
李銳華%徐小倩%張小彊%李英%吳雲%丁崗%王振中%蕭偉
리예화%서소천%장소강%리영%오운%정강%왕진중%소위
高效液相色谱法%朝藿定A%朝藿定B%朝藿定C%淫羊藿苷%宝霍苷I
高效液相色譜法%朝藿定A%朝藿定B%朝藿定C%淫羊藿苷%寶霍苷I
고효액상색보법%조곽정A%조곽정B%조곽정C%음양곽감%보곽감I
HPLC%epimedinA%epimedinB%epimedinC%epimedium glycoside%baohuosideI
目的：建立同时测定淫羊藿总黄酮胶囊中朝霍定A、朝霍定B、朝霍定C、淫羊藿苷、宝霍苷I等5种成分含量的高效液相色谱（HPLC）法。方法：所采用的色谱条件为：色谱柱：Eclipse Plus C18（250 mm×4.6 mm，5μm）；流动相：乙腈-水，梯度洗脱；体积流量：1.0 mL·min-1；检测波长：270 nm；柱温：25℃。结果：朝霍定A、朝霍定B、朝霍定C、淫羊藿苷、宝霍苷I分别在3.10-62.00、5.70-114.00、9.14-182.80、15.20-304.00、1.56-31.20μg·mL-1范围内呈良好的线性关系，相关系数r均大于0.9993；平均加样回收率分别为101.06%（RSD=1.05%，n=6）、100.78%（RSD=1.08%，n=6）、99.17%（RSD=1.14%，n=6）、100.23%（RSD=0.68%，n=6）、99.09%（RSD=1.30%，n=6），该方法的精密度、重复性和稳定性良好。结论：该方法简便、准确，为淫羊藿总黄酮胶囊多成分含量测定提供一定的参考价值。
目的：建立同時測定淫羊藿總黃酮膠囊中朝霍定A、朝霍定B、朝霍定C、淫羊藿苷、寶霍苷I等5種成分含量的高效液相色譜（HPLC）法。方法：所採用的色譜條件為：色譜柱：Eclipse Plus C18（250 mm×4.6 mm，5μm）；流動相：乙腈-水，梯度洗脫；體積流量：1.0 mL·min-1；檢測波長：270 nm；柱溫：25℃。結果：朝霍定A、朝霍定B、朝霍定C、淫羊藿苷、寶霍苷I分彆在3.10-62.00、5.70-114.00、9.14-182.80、15.20-304.00、1.56-31.20μg·mL-1範圍內呈良好的線性關繫，相關繫數r均大于0.9993；平均加樣迴收率分彆為101.06%（RSD=1.05%，n=6）、100.78%（RSD=1.08%，n=6）、99.17%（RSD=1.14%，n=6）、100.23%（RSD=0.68%，n=6）、99.09%（RSD=1.30%，n=6），該方法的精密度、重複性和穩定性良好。結論：該方法簡便、準確，為淫羊藿總黃酮膠囊多成分含量測定提供一定的參攷價值。
목적：건립동시측정음양곽총황동효낭중조곽정A、조곽정B、조곽정C、음양곽감、보곽감I등5충성분함량적고효액상색보（HPLC）법。방법：소채용적색보조건위：색보주：Eclipse Plus C18（250 mm×4.6 mm，5μm）；류동상：을정-수，제도세탈；체적류량：1.0 mL·min-1；검측파장：270 nm；주온：25℃。결과：조곽정A、조곽정B、조곽정C、음양곽감、보곽감I분별재3.10-62.00、5.70-114.00、9.14-182.80、15.20-304.00、1.56-31.20μg·mL-1범위내정량호적선성관계，상관계수r균대우0.9993；평균가양회수솔분별위101.06%（RSD=1.05%，n=6）、100.78%（RSD=1.08%，n=6）、99.17%（RSD=1.14%，n=6）、100.23%（RSD=0.68%，n=6）、99.09%（RSD=1.30%，n=6），해방법적정밀도、중복성화은정성량호。결론：해방법간편、준학，위음양곽총황동효낭다성분함량측정제공일정적삼고개치。
This study was aimed to establish an HPLC method for the content determination ofepimedin A, epimedinB,epimedinC,epimedium glycoside,baohuosideI in epimedium flavonoids capsule. The elusion was performed on an Eclipse Plus C18column (250 mm× 4.6 mm, 5μm). The mobile phase was composed of acetonitrile and water with a gradient elution. The flow rate was set at 1 mL·min-1. The detection wave length was set at 270 nm. The column temperature was 25℃. The results showed that the linear ranges of epimedin A,epimedinB,epimedinC,epimedium glycoside,baohuosideI were 3.10-62.00μg·mL-1, 5.70-114.00μg·mL-1, 9.14-182.80μg·mL-1, 15.20-304.00μg·mL-1, and 1.56-31.20μg·mL-1, respectively. The correlation coefficientr was more than 0.999 3. The average recoveries were 101.06% (RSD = 1.05%,n = 6), 100.78% (RSD = 1.08%,n = 6), 99.17% (RSD = 1.14%,n = 6), 100.23% (RSD = 0.68%,n = 6), and 99.09% (RSD = 1.30%,n = 6), respectively. This experiment was precise, reproducible and stable. It was concluded that the method was simple and accurate, which provided a certain reference value for the multi-component assaying of epimedium flavonoids capsule.
