世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
World Science and Technology-Modernization of Traditional Chinese Medicine
2015年
9期
1801-1805
,共5页
郭亚春%梁秀军%高亚贤%宋鸿儒
郭亞春%樑秀軍%高亞賢%宋鴻儒
곽아춘%량수군%고아현%송홍유
血管内皮生长因子%PCR%薯蓣皂苷元%c-fos%c-jun
血管內皮生長因子%PCR%藷蕷皂苷元%c-fos%c-jun
혈관내피생장인자%PCR%서여조감원%c-fos%c-jun
Vascular endothelial growth factor%PCR%diosgenin%c-fos%c-jun
目的：通过观察中药薯蓣皂苷元对牛Ⅱ型胶原诱导型关节炎（Collagen-Induced Arthritis， CIA）大鼠膝关节滑膜激活蛋白-1（Activator Protein-1，AP-1），血管内皮生长因子（Vascular Endothelial Growth Factor，VEGF）的影响，探讨中药薯蓣皂苷元治疗类风湿关节炎的可能作用机制。方法：通过建立大鼠CIA模型，实验随机分空白对照组、CIA模型组、中药薯蓣皂苷元组和阳性药物对照组（雷公藤多苷组），采用原位杂交方法检测膝关节滑膜组织AP-1（c-fos和c-jun）的表达情况，应用Real-Time PCR方法检测大鼠膝关节滑膜组织VEGF mRNA的表达情况。结果：CIA模型组大鼠膝关节滑膜组织c-jun、c-fos和VEGF mRNA表达明显高于空白对照组；应用中药薯蓣皂苷元及雷公藤治疗后，c-jun、c-fos和VEGF mRNA表达明显降低。结论：薯蓣皂苷元可能通过对AP-1的两个亚单位c-jun和c-fos的调节来影响滑膜组织VEGF的表达，进而抑制滑膜血管新生，从而对类风湿关节炎发挥治疗作用。
目的：通過觀察中藥藷蕷皂苷元對牛Ⅱ型膠原誘導型關節炎（Collagen-Induced Arthritis， CIA）大鼠膝關節滑膜激活蛋白-1（Activator Protein-1，AP-1），血管內皮生長因子（Vascular Endothelial Growth Factor，VEGF）的影響，探討中藥藷蕷皂苷元治療類風濕關節炎的可能作用機製。方法：通過建立大鼠CIA模型，實驗隨機分空白對照組、CIA模型組、中藥藷蕷皂苷元組和暘性藥物對照組（雷公籐多苷組），採用原位雜交方法檢測膝關節滑膜組織AP-1（c-fos和c-jun）的錶達情況，應用Real-Time PCR方法檢測大鼠膝關節滑膜組織VEGF mRNA的錶達情況。結果：CIA模型組大鼠膝關節滑膜組織c-jun、c-fos和VEGF mRNA錶達明顯高于空白對照組；應用中藥藷蕷皂苷元及雷公籐治療後，c-jun、c-fos和VEGF mRNA錶達明顯降低。結論：藷蕷皂苷元可能通過對AP-1的兩箇亞單位c-jun和c-fos的調節來影響滑膜組織VEGF的錶達，進而抑製滑膜血管新生，從而對類風濕關節炎髮揮治療作用。
목적：통과관찰중약서여조감원대우Ⅱ형효원유도형관절염（Collagen-Induced Arthritis， CIA）대서슬관절활막격활단백-1（Activator Protein-1，AP-1），혈관내피생장인자（Vascular Endothelial Growth Factor，VEGF）적영향，탐토중약서여조감원치료류풍습관절염적가능작용궤제。방법：통과건립대서CIA모형，실험수궤분공백대조조、CIA모형조、중약서여조감원조화양성약물대조조（뢰공등다감조），채용원위잡교방법검측슬관절활막조직AP-1（c-fos화c-jun）적표체정황，응용Real-Time PCR방법검측대서슬관절활막조직VEGF mRNA적표체정황。결과：CIA모형조대서슬관절활막조직c-jun、c-fos화VEGF mRNA표체명현고우공백대조조；응용중약서여조감원급뢰공등치료후，c-jun、c-fos화VEGF mRNA표체명현강저。결론：서여조감원가능통과대AP-1적량개아단위c-jun화c-fos적조절래영향활막조직VEGF적표체，진이억제활막혈관신생，종이대류풍습관절염발휘치료작용。
This paper was aimed to study effects of diosgenin on expressions of activator protein-1 (AP-1) and vascular endothelial growth factor (VEGF) in synovial tissues of collagen-induced arthritis (CIA) rats induced by bovine type II collagen, in order to investigate the possible mechanism of herbal medicine diosgenin in the treatment of rheumatoid arthritis (RA). After the CIA rats models were successfully established, rats were randomly divided into the blank control group, CIA model group, diosgenin group, and positive medicine control (tripterygium) group. The in situ hybridization was used to detect the expressions of AP-1 (c-fos and c-jun) in synovial tissues of the knee joint. The real-time PCR was used to detect the VEGF mRNA expression in synovial tissues of rats’ knee joints. The results showed that c-jun and c-fos, VEGF mRNA expressions in synovial tissues of rats’ knee joints were obviously higher than that of the blank control group (P<0.01). After treatment of diosgenin and tripterygium, the expressions of c-jun and c-fos, VEGF mRNA were significantly reduced (P<0.01). It was concluded that diosgenin may regulate the expression of VEGF in synovial tissues through c-jun and c-fos of AP-1 in order to inhibit synovial angiogenesis for the treatment of RA.
