CAJ | 학술논문

目的：建立从金银花中分离制备新绿原酸对照品的方法。方法：利用HPD200A大孔树脂对金银花水提液中的新绿原酸富集，经中低压制备色谱分离制备金银花中新绿原酸单体，运用HPLC对新绿原酸进行含量测定。采用现代波谱技术ESI-MS、1H-NMR、13C-NMR进行结构鉴定。结果：新绿原酸的最佳纯化工艺为加5 BV水洗脱，收集水洗液，浓缩、干燥，得新绿原酸粗品；以乙腈-0.5%甲酸溶液（10：90）为流动相，流速20 mL?min-1，检测波长326 nm，进行分离纯化，分离制备的新绿原酸的纯度达98.86%，收率为89.1%。结论：该方法可有效制备高纯度的新绿原酸对照品，以用于中药新药中定性鉴别和含量测定。
목적：건립종금은화중분리제비신록원산대조품적방법。방법：이용HPD200A대공수지대금은화수제액중적신록원산부집，경중저압제비색보분리제비금은화중신록원산단체，운용HPLC대신록원산진행함량측정。채용현대파보기술ESI-MS、1H-NMR、13C-NMR진행결구감정。결과：신록원산적최가순화공예위가5 BV수세탈，수집수세액，농축、간조，득신록원산조품；이을정-0.5%갑산용액（10：90）위류동상，류속20 mL?min-1，검측파장326 nm，진행분리순화，분리제비적신록원산적순도체98.86%，수솔위89.1%。결론：해방법가유효제비고순도적신록원산대조품，이용우중약신약중정성감별화함량측정。
This study was aimed to establish a separation method for neochlorogenic acid reference substances from Lonicera japonica. Refined neochlorogenic acid inL. japonica water extract was separated and concentrated by HPD200A macroporous resin, which was isolated and purified by medium-low-pressure preparative chromatography and determined by HPLC. The structure was identified by various spectroscopic data including ESI-MS,1H-NMR and13C-NMR. The results showed that the optimal purification technology conditions were as follows: washed with 5BV of water, collected elution, concentration, drying; neochlorogenic acid crude products were eluted with acetonitrile-0.5% formic acid solution (10:90) with the flow rate of 20 mL·min-1; and the detection wavelength was 326 nm. The contents of the prepared neochlorogenic acid reached to 98.86% and the yield was 89.1%. It was concluded that the method was effective for the preparation of neochlorogenic acid with high purity. It can be used to prepare the reference substances for quantitative analysis and content determination of Chinese materia medica.