世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
World Science and Technology-Modernization of Traditional Chinese Medicine
2015年
9期
1876-1882
,共7页
王凤云%韩亮%赖小平
王鳳雲%韓亮%賴小平
왕봉운%한량%뢰소평
燕窝%DNA条形码%Cytb序列%基源鉴定
燕窩%DNA條形碼%Cytb序列%基源鑒定
연와%DNA조형마%Cytb서렬%기원감정
Edible bird’s nest%DNA barcoding%Cytb%genetic identification
目的：利用DNA条形码鉴定技术，快速、准确地鉴别不同种类及产地的商品燕窝，揭示其不同基源的物种差异。方法：采用试剂盒提取基因组DNA，利用PCR技术扩增Cytb序列，应用DNAStar软件进行校对拼接，采用MEGA 6.0软件对32份样品的条形码序列进行比对，构建邻接树（Neighbor-Joining tree，NJ），并进行遗传距离分析。结果：实验所用的Cytb条形码可鉴别出燕窝的物种及亚种；收集的32个商品燕窝中，23个官燕窝的基源为爪哇金丝燕Aerodramus fuciphagus，8个官燕窝的基源为其亚种淡腰金丝燕Aerodramus fuciphagus germani，而1个毛燕窝的基源为大金丝燕Aerodramus maximus或其亚种Aerodramus maximus lowi。结论：不同来源的商品燕窝基源差异较大，利用Cytb条形码可快速、准确鉴别出燕窝基源。
目的：利用DNA條形碼鑒定技術，快速、準確地鑒彆不同種類及產地的商品燕窩，揭示其不同基源的物種差異。方法：採用試劑盒提取基因組DNA，利用PCR技術擴增Cytb序列，應用DNAStar軟件進行校對拼接，採用MEGA 6.0軟件對32份樣品的條形碼序列進行比對，構建鄰接樹（Neighbor-Joining tree，NJ），併進行遺傳距離分析。結果：實驗所用的Cytb條形碼可鑒彆齣燕窩的物種及亞種；收集的32箇商品燕窩中，23箇官燕窩的基源為爪哇金絲燕Aerodramus fuciphagus，8箇官燕窩的基源為其亞種淡腰金絲燕Aerodramus fuciphagus germani，而1箇毛燕窩的基源為大金絲燕Aerodramus maximus或其亞種Aerodramus maximus lowi。結論：不同來源的商品燕窩基源差異較大，利用Cytb條形碼可快速、準確鑒彆齣燕窩基源。
목적：이용DNA조형마감정기술，쾌속、준학지감별불동충류급산지적상품연와，게시기불동기원적물충차이。방법：채용시제합제취기인조DNA，이용PCR기술확증Cytb서렬，응용DNAStar연건진행교대병접，채용MEGA 6.0연건대32빈양품적조형마서렬진행비대，구건린접수（Neighbor-Joining tree，NJ），병진행유전거리분석。결과：실험소용적Cytb조형마가감별출연와적물충급아충；수집적32개상품연와중，23개관연와적기원위조왜금사연Aerodramus fuciphagus，8개관연와적기원위기아충담요금사연Aerodramus fuciphagus germani，이1개모연와적기원위대금사연Aerodramus maximus혹기아충Aerodramus maximus lowi。결론：불동래원적상품연와기원차이교대，이용Cytb조형마가쾌속、준학감별출연와기원。
This study was aimed to quickly and accurately identify different origins and categories of commodity edible bird’s nest (EBN) using DNA barcoding technique, in order to reveal its genetic differences. The total genomic DNA was isolated from the EBN samples. And Cytb gene sequences were amplified and sequenced by PCR. Then, 32 sequences were aligned and analyzed with DNAStar and MEGA 6.0 software. NJ phylogenetic tree was constructed. The nearest distance was calculated. The results showed that the original species of 32 samples of EBN were identified.Aerodramus fuciphagus was the genetic origin of 23 white nest samples. AndAerodramus fuciphagus germaniwas the genetic origin of the other 8 samples. The origin of black nest sample wasAerodramus maximusorAerodramus maximus lowi. It was concluded that the genetic origin of different EBN categories was variant. The identification of EBN’s origin species with Cytb sequence was quick and accurate.
