CAJ | 학술논문

目的：建立测定藤黄科植物中异藤黄酚含量的高效液相色谱分析方法（HPLC）。方法：使用岛津Inertsil WP300 C18填料色谱柱（4.6 mm×150 mm，5μm），流动相为甲醇?水=75?25，流速为1.0 mL·min-1，柱温为40℃，检测波长γ为277 nm。结果：异藤黄酚的线性范围为0.0057-0.0399μg，平均加样回收率为99.58%，相对标准偏差（RSD）为1.25%。山竹子、岭南山竹子、单花山竹子、黄牛木和薄叶红厚壳中异藤黄酚（Isogarcinol）的含量分别为0.285%，0.199%，0.857%，0.161%和0.006%；而越南黄牛木和红厚壳中没检测出异藤黄酚。结论：该方法简便、准确、灵敏度高，稳定性和重复性好，可用于中药材中异藤黄酚含量的测定。
목적：건립측정등황과식물중이등황분함량적고효액상색보분석방법（HPLC）。방법：사용도진Inertsil WP300 C18전료색보주（4.6 mm×150 mm，5μm），류동상위갑순?수=75?25，류속위1.0 mL·min-1，주온위40℃，검측파장γ위277 nm。결과：이등황분적선성범위위0.0057-0.0399μg，평균가양회수솔위99.58%，상대표준편차（RSD）위1.25%。산죽자、령남산죽자、단화산죽자、황우목화박협홍후각중이등황분（Isogarcinol）적함량분별위0.285%，0.199%，0.857%，0.161%화0.006%；이월남황우목화홍후각중몰검측출이등황분。결론：해방법간편、준학、령민도고，은정성화중복성호，가용우중약재중이등황분함량적측정。
This study was aimed to establish a HPLC method for the determination of isogarcinol. Daojin Inertsil WP300 C18 (4.6 mm× 150 mm, 5μm) was employed with methanol and water (75?25) as the mobile phase. The flow rate was 1.0 mL·min-1. The column temperature was set at 40°C. The detection wavelengthγ was set at 277 nm. The results showed that the linear range of isogarcinol was 0.005 7-0.039 9μg. The average recovery rate was 99.58%. The relative standard deviation (RSD) was 1.25%. The contents of isogarcinol inGarcinia mangostana,Garcinia oblongifolia,Garcinia oligantha,Cratoxylum cochinchinense andCalophyllum membranaceum were 0.285%, 0.199%, 0.857%, 0.161% and 0.006%, respectively. Isogarcinol was not detected inCratoxylum formosum orCalophyllum inophyllum. It was concluded that the method was convenient, accurate with high sensitivity, good stability and repeatability. It can be used for determination of isogarcinol content in Chinese herbal medicine.