世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
World Science and Technology-Modernization of Traditional Chinese Medicine
2015年
9期
1953-1958
,共6页
金莲花%药渣%发酵提取物%白色念珠菌%阴道炎
金蓮花%藥渣%髮酵提取物%白色唸珠菌%陰道炎
금연화%약사%발효제취물%백색념주균%음도염
Globeflower%residue%fermentation extract%C. albicans%vaginitis
目的：观察金莲花药渣发酵提取物（Extraction of Globeflower Residue Fermentation，EGRF）体外对白色念珠菌抑杀效果及体内对小鼠念珠菌性阴道炎的治疗效果。方法：通过试管法配合平皿法检测EGRF对白色念珠菌的最小抑菌浓度（Minimum Inhibitory Concentration，MIC）和最小杀菌浓度（Minimum Bactericidal Concentration，MBC）；构建小鼠白念珠菌性阴道炎模型，将造模成功小鼠随机分为正常对照组，模型组，EGRF低、中、高剂量组（EGRF给药浓度分别为：40、80、160 mg?kg-1），氟康唑组（20 mg?kg-1），共6组。各药均阴道内给药，每天1次，连续给药7天，观察小鼠外阴炎症反应程度、阴道念珠菌转阴率、阴道灌洗液载菌量和阴道粘膜的病理改变。结果：EGRF对白色念珠菌的MIC为0.31 mg?mL-1，对白色念珠菌的MBC为1.25 mg?mL-1，EGRF和氟康唑的效价单位比MIC为2?1，MBC为1?1。与模型组相比，EGRF及氟康唑治疗组的小鼠外阴炎症反应程度减轻及分泌物中念珠菌数量随着给药时间增加而显著减少；小鼠阴道灌洗液白色念珠菌转阴率增加，载菌量明显减少；小鼠阴道组织病理改变有不同程度的减轻，有明显剂量依赖性，高剂量组与氟康唑组相近。结论：EGRF于体外对白色念珠菌抑杀作用显著，对小鼠白色念珠菌性阴道炎疗效较好。
目的：觀察金蓮花藥渣髮酵提取物（Extraction of Globeflower Residue Fermentation，EGRF）體外對白色唸珠菌抑殺效果及體內對小鼠唸珠菌性陰道炎的治療效果。方法：通過試管法配閤平皿法檢測EGRF對白色唸珠菌的最小抑菌濃度（Minimum Inhibitory Concentration，MIC）和最小殺菌濃度（Minimum Bactericidal Concentration，MBC）；構建小鼠白唸珠菌性陰道炎模型，將造模成功小鼠隨機分為正常對照組，模型組，EGRF低、中、高劑量組（EGRF給藥濃度分彆為：40、80、160 mg?kg-1），氟康唑組（20 mg?kg-1），共6組。各藥均陰道內給藥，每天1次，連續給藥7天，觀察小鼠外陰炎癥反應程度、陰道唸珠菌轉陰率、陰道灌洗液載菌量和陰道粘膜的病理改變。結果：EGRF對白色唸珠菌的MIC為0.31 mg?mL-1，對白色唸珠菌的MBC為1.25 mg?mL-1，EGRF和氟康唑的效價單位比MIC為2?1，MBC為1?1。與模型組相比，EGRF及氟康唑治療組的小鼠外陰炎癥反應程度減輕及分泌物中唸珠菌數量隨著給藥時間增加而顯著減少；小鼠陰道灌洗液白色唸珠菌轉陰率增加，載菌量明顯減少；小鼠陰道組織病理改變有不同程度的減輕，有明顯劑量依賴性，高劑量組與氟康唑組相近。結論：EGRF于體外對白色唸珠菌抑殺作用顯著，對小鼠白色唸珠菌性陰道炎療效較好。
목적：관찰금연화약사발효제취물（Extraction of Globeflower Residue Fermentation，EGRF）체외대백색념주균억살효과급체내대소서념주균성음도염적치료효과。방법：통과시관법배합평명법검측EGRF대백색념주균적최소억균농도（Minimum Inhibitory Concentration，MIC）화최소살균농도（Minimum Bactericidal Concentration，MBC）；구건소서백념주균성음도염모형，장조모성공소서수궤분위정상대조조，모형조，EGRF저、중、고제량조（EGRF급약농도분별위：40、80、160 mg?kg-1），불강서조（20 mg?kg-1），공6조。각약균음도내급약，매천1차，련속급약7천，관찰소서외음염증반응정도、음도념주균전음솔、음도관세액재균량화음도점막적병리개변。결과：EGRF대백색념주균적MIC위0.31 mg?mL-1，대백색념주균적MBC위1.25 mg?mL-1，EGRF화불강서적효개단위비MIC위2?1，MBC위1?1。여모형조상비，EGRF급불강서치료조적소서외음염증반응정도감경급분비물중념주균수량수착급약시간증가이현저감소；소서음도관세액백색념주균전음솔증가，재균량명현감소；소서음도조직병리개변유불동정도적감경，유명현제량의뢰성，고제량조여불강서조상근。결론：EGRF우체외대백색념주균억살작용현저，대소서백색념주균성음도염료효교호。
This study was aimed to observe the antibacterial activity and bactericidal action ofC.albicans in vitro, and the effects of curing monilial vaginitis mouse by extraction of globeflower residue fermentation (EGRF) in vivo.In vitrostudy, test tube method as well as plate method were used to determine the minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) ofC.albicansrespectively.In vivo, mouse were devided into normal controlled group,C. albicans vaginitis model group (Model), Model + EGRF (40, 80, 160 mg?kg-1) group, and fluconazole group (20 mg?kg-1). All drugs were vaginal delivery once a day with continuous administration for seven days. Then vulva inflammation, negative rate of vaginal discharge, microbial load of vaginal lavage and the pathological changes of vaginal mucosa were observed. After the treatment of EGRF, the MIC and MBC of Candida albicans were 0.31 mg?mL-1 and 1.25 mg?mL-1, respectively, while the potency unit ratios between EGRF and fluconazole of MIC and MBC were 2 to 1 and 1 to 1, respectively. In comparison with Model, vulva inflammation of Model + EGRF gourp and fluconazole group was improved, whileC. albicanscount in vaginal secretions of these two groups were decreased, the overcast rate ofC. albicansof vaginal douche was increased, and pathological changes of vaginal mucosa were also improved in the two groups, which were in dose-dependent manners. And high dose Model + EGRF group was close to fluconazole group. In conclusion, EGRF had obvious inhibitory effect onC. albicans in vitro. It also had a better therapeutic effect onC. albicans vaginitis mouse.
