CAJ | 학술논문

目的：探讨雌雄激素联合作用对高脂血症小鼠血脂水平及凝血功能的影响。方法对小鼠行去势手术后通过高脂饮食建立高脂血症模型，通过给予不同激素将老鼠分为5组。造模结束后采集小鼠血液，一部分小鼠分离血清检测小鼠血脂水平，一部分小鼠分离血浆检测凝血功能。结果高脂饮食后，小鼠血清总胆固醇、甘油三酯和低密度脂蛋白水平较对照组相比明显升高，凝血酶原时间、活化部分凝血酶原时间时间明显缩短（P＜0.05），给予雌雄激素联合治疗后，与造模组相比，小鼠血清总胆固醇、甘油三酯和低密度脂蛋白水平较对照组相比明显下降，凝血酶原时间、活化部分凝血酶原时间时间明显延长（P＜0.05），而与对照组无明显差异。结论雌雄激素联合治疗可以调节血脂，显著降低低密度脂蛋白的水平，调节凝血功能，提示雌雄激素联合作用可以降低冠心病的发病风险，为冠心病的激素治疗提供新思路。
목적：탐토자웅격소연합작용대고지혈증소서혈지수평급응혈공능적영향。방법대소서행거세수술후통과고지음식건립고지혈증모형，통과급여불동격소장로서분위5조。조모결속후채집소서혈액，일부분소서분리혈청검측소서혈지수평，일부분소서분리혈장검측응혈공능。결과고지음식후，소서혈청총담고순、감유삼지화저밀도지단백수평교대조조상비명현승고，응혈매원시간、활화부분응혈매원시간시간명현축단（P＜0.05），급여자웅격소연합치료후，여조모조상비，소서혈청총담고순、감유삼지화저밀도지단백수평교대조조상비명현하강，응혈매원시간、활화부분응혈매원시간시간명현연장（P＜0.05），이여대조조무명현차이。결론자웅격소연합치료가이조절혈지，현저강저저밀도지단백적수평，조절응혈공능，제시자웅격소연합작용가이강저관심병적발병풍험，위관심병적격소치료제공신사로。
Objective To investigate the influence of synergistic effect of estradiol and testosterone on the level of lipids and coagulation function in mice with hyperlipidemia .Methods We established a maouse model in hyperlipidmia , giving estradiol (1 μg/d), testosterone (7 μg/d) or estradiol combined testosterone (1 μg/d E2 +7Tμg/d), respectively.After 14 weeks, we collected the blood from the mice , separated the serum to detect the lipids level , separated the plasma to test the coagulation function .Result Compared with controls , after high-fat diet , the serum level of total cholesterol ( TC) , triglyceride ( TG) , high density lipoprotein ( HDL) were significantly increased , PT and APTT were shorter ( P<0.05 ).However, after treating with estradiol combined testosterone , compared with cases, the serum level of C, TG and HDL were lower and PT , APTT were longer ( P <0.05 ), had no significance with controls . Conclusion The synergistic effect of estradiol and testosterone can mediate the lipids , reduce the level of LDL , regulate the coagulation function , reduce the risk of coronary heart disease , and also provide a new strategy for hormone therapy in coronary heart disease .