CAJ | 학술논문

目的：探讨姜黄素对沙漠干热环境下大鼠生存率的影响。方法选择SPF级6～8周龄雄性SD大鼠40只，随机分为对照组、姜黄素低剂量预处理组、姜黄素中剂量预处理组、姜黄素高剂量预处理组、溶剂组。对照组，正常饲喂；低剂量组、中剂量组、高剂量组、溶剂组分别等体积灌胃连续7d。置于西北地区特殊环境人工实验舱，设定沙漠干热环境气候模式（温度41℃，湿度10％，紫外线辐射度40W／m2）。观察各组大鼠机体的生命体征并每半小时监测核心体温变化，记录死亡时间。结果五组大鼠从被置于沙漠干热环境开始，0～30 min，姜黄素三个剂量组的核心体温与对照组、溶剂组比较无显著差异（ P＞0.05）。从60 min开始，60 min、90 min、120 min、150 min、180 min、210 min、240 min7个时间点，低剂量组、中剂量组、高剂量组的核心体温低于对照组、溶剂组，差异有显著性（P＜0.05）。从0～240 min各时间点低剂量组、中剂量组、高剂量组组间比较均无显著性差异（P＞0.05）。对照组、姜黄素低、中、高剂量治疗组及溶剂组五组大鼠平均生存时间分别为（222.95±28.23） min、（231.35±40.96）min、（255.00±30.39）min、（263.80±56.71）min、（223.00±27.23）min。中、高剂量姜黄素预处理组大鼠的生存时间均明显长于低浓度预处理组、对照组和溶剂组（P＜0.05），其中高剂量组效果显著（P＜0.01）。结论姜黄素预处理能够提高沙漠干热环境大鼠的生存时间，提示姜黄素可能在提高沙漠干热环境大鼠耐热性方面发挥重要作用，姜黄素可能在沙漠干热环境中暑的防治方面具有潜在的临床应用价值。
목적：탐토강황소대사막간열배경하대서생존솔적영향。방법선택SPF급6～8주령웅성SD대서40지，수궤분위대조조、강황소저제량예처리조、강황소중제량예처리조、강황소고제량예처리조、용제조。대조조，정상사위；저제량조、중제량조、고제량조、용제조분별등체적관위련속7d。치우서북지구특수배경인공실험창，설정사막간열배경기후모식（온도41℃，습도10％，자외선복사도40W／m2）。관찰각조대서궤체적생명체정병매반소시감측핵심체온변화，기록사망시간。결과오조대서종피치우사막간열배경개시，0～30 min，강황소삼개제량조적핵심체온여대조조、용제조비교무현저차이（ P＞0.05）。종60 min개시，60 min、90 min、120 min、150 min、180 min、210 min、240 min7개시간점，저제량조、중제량조、고제량조적핵심체온저우대조조、용제조，차이유현저성（P＜0.05）。종0～240 min각시간점저제량조、중제량조、고제량조조간비교균무현저성차이（P＞0.05）。대조조、강황소저、중、고제량치료조급용제조오조대서평균생존시간분별위（222.95±28.23） min、（231.35±40.96）min、（255.00±30.39）min、（263.80±56.71）min、（223.00±27.23）min。중、고제량강황소예처리조대서적생존시간균명현장우저농도예처리조、대조조화용제조（P＜0.05），기중고제량조효과현저（P＜0.01）。결론강황소예처리능구제고사막간열배경대서적생존시간，제시강황소가능재제고사막간열배경대서내열성방면발휘중요작용，강황소가능재사막간열배경중서적방치방면구유잠재적림상응용개치。
Objective To study the effects of curcumin on the survival rate of the rats in the dry heat environment of desert.Methods The 40 selected 6~8 week-old male SPF grade SD rats were randomly divided into control group , low-dose curcumin pretreated group , middle-dose curcumin pretreated group , high-dose curcumin pretreated group and solvent group .The control group were fed as normal , the three curvumin pretreated group were gavaged with different dose of curcumin for 7 consecutive days , the solvent group were gavaged equal volume of solvent for 7 consecutive days .Then all the rats were put into the dry heat environment (temperature of 41℃, humidity 10%,ultraviolet radiation 40W/m2) in The Simulated Climate Cabin for Special Environment of Northwest of China , The vital signs of the rats were observed , The core body temperature of the rats were monitored for every half an hour , the dead time were recorded .Results The core body temperature of the five groups had no significant different at the first half hour after the started in the dry heat desert environment (P >0.05).However, after 60 min from the beginning, the core body temperature of the three curcumin pretreated group were significantly lower than the control group and the solvent group at the 7 time point of 60 min, 90 min, 120 min, 150 min, 180 min, 210 min, 240 min.The core body temperature had no significant different in the three curcumin pretreated group at each time point from the beginning to 240 min (P >0.05).The survival time for the control group, low-dose curcumin pretreated group , middle-dose curcumin pretreated group , high-dose curcumin pretreated group and solvent group were (222.95 ±28.23)min,(231.35 ±40.96)min,(255.00 ±30.39)min,(263.80 ±56.71) min,(223.00 ±27.23)min, respectively.The survival time of the middle-dose curcumin pretreated group and high-dose curcumin pretreated group were significantly longer than the low-dose curcumin pretreated group , control group and the solvent group(P <0.05), and the high-dose curcumin pretreated group had the most obvious effects on the survival time compared with the other two curcumin pretreated group (P <0.01).Conclusion Curcumin could increase the survival time of rats in the dry heat environment of the desert , which indicated that the curcumin may exert its heat tolerance effects in dry heat environment , curcumin may have potential clinical value in the prevention and treatment for the heatstrok in dry heat environment of desert .