CAJ | 학술논문

目的：利用高效液相色谱建立三军丸的甲醇超声提取的指纹图谱，为三军丸的质量控制和评价提供可靠的方法。方法采用 HPLC 梯度洗脱技术进行分离分析，采用 Venusil XBP C18（4.6 mm ×250 nm），流动相甲醇：乙腈（3：1）-0.1%磷酸，流速为1.0 ml/ min，紫外检测器（λ=280 nm），柱温35℃。结果10个批次的三军丸之间有11个共有指纹峰，大多数的峰可以达到较好的分离效果，建立的 HPLC 指纹图谱具有较高的重现性。结论建立的三军丸高效液相指纹图谱方法稳定、可靠，为三军丸质量控制提供了简单、可靠的依据，可以作为三军丸鉴别和质量控制的有效方法。
목적：이용고효액상색보건립삼군환적갑순초성제취적지문도보，위삼군환적질량공제화평개제공가고적방법。방법채용 HPLC 제도세탈기술진행분리분석，채용 Venusil XBP C18（4.6 mm ×250 nm），류동상갑순：을정（3：1）-0.1%린산，류속위1.0 ml/ min，자외검측기（λ=280 nm），주온35℃。결과10개비차적삼군환지간유11개공유지문봉，대다수적봉가이체도교호적분리효과，건립적 HPLC 지문도보구유교고적중현성。결론건립적삼군환고효액상지문도보방법은정、가고，위삼군환질량공제제공료간단、가고적의거，가이작위삼군환감별화질량공제적유효방법。
Objective To establish HPLC fingerprints of Sanjun Pill and to provide a foundation for the identification of its quality. Methods A gradient separated method was applied. Venusil XBP C18(4. 6 mm × 250 nm),mobile phase was methanol:acetonitrile(3: 1)- 0. 1% phosphoric acid,detection wavelength was at 280 nm,flow rate was 1. 0 ml/ min,column temperature was at 35 ℃ . Results Eleven mutual peaks in its preparations were separated effectively. The methodological evaluation showed that this method had a good repeatability. Conclusion The results of peak areas are in accordance with the request of fingerprint. The established fingerprint can be used for the quality control.