世界中医药
世界中醫藥
세계중의약
World Chinese Medicine
2015年
10期
1582-1585
,共4页
赵刚%夏莹%黄志军%裴学军%唐惠丹
趙剛%夏瑩%黃誌軍%裴學軍%唐惠丹
조강%하형%황지군%배학군%당혜단
健脾生血颗粒%大鼠%胚胎-胎仔发育毒性
健脾生血顆粒%大鼠%胚胎-胎仔髮育毒性
건비생혈과립%대서%배태-태자발육독성
Invigorating Spleen-Generating Blood Granule%Rat%Toxicity on embryo-fetal development
目的：评价灌胃给予健脾生血颗粒对大鼠胚胎-胎仔发毒性作用。方法：孕鼠随机分为阴性对照、阳性对照和低、中、高剂量试验组，分别于孕期第6至第15天连续灌胃给药。其中，低、中、高剂量试验组分别灌胃健脾生血颗粒浸膏1．16、3．48、10．44 g／kg／d，阳性对照组灌胃维生素 A 醋酸酯0．08 g／kg／d，阴性对照组给予同样体积的纯净水。记录孕鼠的体重、身长、摄食量及临床表现。于妊娠第20天剖检孕鼠，观察胎盘和胎鼠外观形态，记录黄体数、活胎数、死胎数、吸收胎、着床数及胎盘质量，制作骨骼和内脏标本，以作进一步检查。结果：阳性对照组出现了明显的致畸作用，证明本试验系统结果可靠。试验组大鼠经给药后，均未见明显异常临床症状，各剂量组对胚胎的形成均无明显影响，且黄体数、植入数、死胎、活胎以及吸收胎与阴性对照组比较差异均无统计学意义。此外，低、中剂量的浸膏对亲代大鼠未见明显一般毒性，但高剂量浸膏可致亲代大鼠体重和摄食量下降，影响部分胎鼠的发育，主要表现为体重减轻，少数动物关节畸形等。结论：在本试验条件下，健脾生血颗粒浸膏对大鼠胚胎-胎仔发育毒性的无不良反应剂量为3．48 g／kg，相当于成人临床最大用量的42倍。
目的：評價灌胃給予健脾生血顆粒對大鼠胚胎-胎仔髮毒性作用。方法：孕鼠隨機分為陰性對照、暘性對照和低、中、高劑量試驗組，分彆于孕期第6至第15天連續灌胃給藥。其中，低、中、高劑量試驗組分彆灌胃健脾生血顆粒浸膏1．16、3．48、10．44 g／kg／d，暘性對照組灌胃維生素 A 醋痠酯0．08 g／kg／d，陰性對照組給予同樣體積的純淨水。記錄孕鼠的體重、身長、攝食量及臨床錶現。于妊娠第20天剖檢孕鼠，觀察胎盤和胎鼠外觀形態，記錄黃體數、活胎數、死胎數、吸收胎、著床數及胎盤質量，製作骨骼和內髒標本，以作進一步檢查。結果：暘性對照組齣現瞭明顯的緻畸作用，證明本試驗繫統結果可靠。試驗組大鼠經給藥後，均未見明顯異常臨床癥狀，各劑量組對胚胎的形成均無明顯影響，且黃體數、植入數、死胎、活胎以及吸收胎與陰性對照組比較差異均無統計學意義。此外，低、中劑量的浸膏對親代大鼠未見明顯一般毒性，但高劑量浸膏可緻親代大鼠體重和攝食量下降，影響部分胎鼠的髮育，主要錶現為體重減輕，少數動物關節畸形等。結論：在本試驗條件下，健脾生血顆粒浸膏對大鼠胚胎-胎仔髮育毒性的無不良反應劑量為3．48 g／kg，相噹于成人臨床最大用量的42倍。
목적：평개관위급여건비생혈과립대대서배태-태자발독성작용。방법：잉서수궤분위음성대조、양성대조화저、중、고제량시험조，분별우잉기제6지제15천련속관위급약。기중，저、중、고제량시험조분별관위건비생혈과립침고1．16、3．48、10．44 g／kg／d，양성대조조관위유생소 A 작산지0．08 g／kg／d，음성대조조급여동양체적적순정수。기록잉서적체중、신장、섭식량급림상표현。우임신제20천부검잉서，관찰태반화태서외관형태，기록황체수、활태수、사태수、흡수태、착상수급태반질량，제작골격화내장표본，이작진일보검사。결과：양성대조조출현료명현적치기작용，증명본시험계통결과가고。시험조대서경급약후，균미견명현이상림상증상，각제량조대배태적형성균무명현영향，차황체수、식입수、사태、활태이급흡수태여음성대조조비교차이균무통계학의의。차외，저、중제량적침고대친대대서미견명현일반독성，단고제량침고가치친대대서체중화섭식량하강，영향부분태서적발육，주요표현위체중감경，소수동물관절기형등。결론：재본시험조건하，건비생혈과립침고대대서배태-태자발육독성적무불량반응제량위3．48 g／kg，상당우성인림상최대용량적42배。
Objective:To investigate the effects of Invigorating Spleen-Generating Blood Granule on embryo-fetal development of rats after intragastric administration.Methods:Pregnant rats were randomly divided into five groups:negative control,positive control,treatment groups with low,medium,or high dosage.The control groups were given oral administration with extract of the granule at 1.16、3.48、10.44 g/kg/d respectively for 10 days after six days’pregnancy.The positive control group were given Vita-min A acetate at 0.08 g/kg/d;the negative group were given pure water with same volume.Then the weight,body length,food consumption,and clinical features of pregnant rats were recorded.Pregnant rats were dissected at their 20 days’pregnancy.Then placentas,fetal rat appearances,skeleton,viscera,and numbers of corpus luteum,live fetus,dying fetus,absorbed fetus,im-plantation and other factors were recorded.Results:Obvious teratogenesis appeared in positive control rats,showed the reliability of the experiment.No abnormal clinical symptoms were observed in the rats of all treatment groups.There were no statistical differ-ences of numbers of corpus luteum,live fetus,dying fetus,absorbed fetus,and implantation among the rats of all treatment groups and negative control.No general toxicity was found from rats with low or medium dosage.But high dosage of extract may result in a decline of weight,and food consumption.Furthermore,the development of fetal rats was also affected.Conclusion:The safe dosage of Invigorating Spleen-Generating Blood Granule on embryo-fetal development of rats was under 3.48 g/kg,42 times of clinical dosage on human.
