中国比较医学杂志
中國比較醫學雜誌
중국비교의학잡지
Chinese Journal of Comparative Medicine
2015年
10期
64-68
,共5页
刘丽君%余柄廷%胡凝珠%孙晓梅%王玮%孙静%胡云章%李建芳
劉麗君%餘柄廷%鬍凝珠%孫曉梅%王瑋%孫靜%鬍雲章%李建芳
류려군%여병정%호응주%손효매%왕위%손정%호운장%리건방
树鼩%粪便细菌%有氧培养%厌氧培养%分离鉴定
樹鼩%糞便細菌%有氧培養%厭氧培養%分離鑒定
수구%분편세균%유양배양%염양배양%분리감정
Tree shrew%Faecal bacteria%Aerobic culture%Anaerobic culture%Isolation identification
目的:了解人工饲养树鼩粪便菌群多样性,为树鼩的正常饲养繁殖和微生物质量控制标准化提供依据。方法随机采集10份树鼩粪便样品,利用有氧及厌氧培养基进行细菌分离培养,提取细菌基因组DNA后PCR扩增16SrRNA基因并测序鉴定。结果本实验从树鼩粪便样品中,经有氧培养分离鉴定出25株、12种细菌,经厌氧培养分离鉴定出25株、10种细菌,包括变形杆菌属、肠球菌属、埃希菌属、志贺菌属、葡萄球菌属、气单胞菌属、拉恩氏菌属、拉乌尔菌属、微小杆菌属、链球菌属、明串珠菌属。结论树鼩肠道好氧菌及厌氧菌具有丰富的种属多样性,普通变形杆菌群、费格森埃希菌群和屎肠球菌群可能是树鼩肠道的主要寄生菌群。
目的:瞭解人工飼養樹鼩糞便菌群多樣性,為樹鼩的正常飼養繁殖和微生物質量控製標準化提供依據。方法隨機採集10份樹鼩糞便樣品,利用有氧及厭氧培養基進行細菌分離培養,提取細菌基因組DNA後PCR擴增16SrRNA基因併測序鑒定。結果本實驗從樹鼩糞便樣品中,經有氧培養分離鑒定齣25株、12種細菌,經厭氧培養分離鑒定齣25株、10種細菌,包括變形桿菌屬、腸毬菌屬、埃希菌屬、誌賀菌屬、葡萄毬菌屬、氣單胞菌屬、拉恩氏菌屬、拉烏爾菌屬、微小桿菌屬、鏈毬菌屬、明串珠菌屬。結論樹鼩腸道好氧菌及厭氧菌具有豐富的種屬多樣性,普通變形桿菌群、費格森埃希菌群和屎腸毬菌群可能是樹鼩腸道的主要寄生菌群。
목적:료해인공사양수구분편균군다양성,위수구적정상사양번식화미생물질량공제표준화제공의거。방법수궤채집10빈수구분편양품,이용유양급염양배양기진행세균분리배양,제취세균기인조DNA후PCR확증16SrRNA기인병측서감정。결과본실험종수구분편양품중,경유양배양분리감정출25주、12충세균,경염양배양분리감정출25주、10충세균,포괄변형간균속、장구균속、애희균속、지하균속、포도구균속、기단포균속、랍은씨균속、랍오이균속、미소간균속、련구균속、명천주균속。결론수구장도호양균급염양균구유봉부적충속다양성,보통변형간균군、비격삼애희균군화시장구균군가능시수구장도적주요기생균군。
Objective Study the fecal flora diversity of the tree shrew , to provide a basis data of fecal bacteria of feeding the tree shrew .Methods Ten tree shrews were used in this study .The Stools of the animals were respectively cultured with oxygen and without oxygen to isolate the bacterial .Then the PCR-amplified 16S rRNA of the bacterial was sequenced and analyzed .Results 25 bacterial strains belonging to ten bacterial species were isolated by anaerobic incubation , and 25 bacterial strains belonging to twelve bacterial species were isolated by aerobic incubation .Proteus vulgaris, Enterococcus faecalis, Escherichia fergusonii, Enterococcus faecium, Shigella flexneri, Shigella sonnei, Staphylococcus aureus , Aeromonas salmonicida subsp .masoucida , Rahnella aquatilis , Exiguobacterium aquaticum , Raoultella terrigena , and Escherichia coli were identified in this study .Conclusions There is a fecal flora diversity of the tree shrew, and the Proteus vulgaris , Escherichia fergusonii and Enterococcus faecium may be the major parasitic flora .