CAJ | 학술논문

采用响应面法优化超声波辅助木聚糖酶联合提取玉米皮中阿魏酸工艺，建立阿魏酸得率与超声温度、超声时间、酶添加量、酶解时间四因素的数学模型，确定玉米皮阿魏酸的最适提取工艺参数；以清除自由基能力评价玉米皮阿魏酸的抗氧化活性。结果表明：玉米皮阿魏酸提取的最佳工艺条件为超声温度33℃、超声时间80 min、酶添加量0.83%、酶解时间65 min、酶解温度50℃，阿魏酸得率为3.47 mg/g。在此优化条件下，玉米皮阿魏酸对DPPH自由基、羟自由基及超氧阴离子自由基均具有较强清除作用，半数抑制浓度分别为0.552、0.282和0.111 mg/mL。
채용향응면법우화초성파보조목취당매연합제취옥미피중아위산공예，건립아위산득솔여초성온도、초성시간、매첨가량、매해시간사인소적수학모형，학정옥미피아위산적최괄제취공예삼수；이청제자유기능력평개옥미피아위산적항양화활성。결과표명：옥미피아위산제취적최가공예조건위초성온도33℃、초성시간80 min、매첨가량0.83%、매해시간65 min、매해온도50℃，아위산득솔위3.47 mg/g。재차우화조건하，옥미피아위산대DPPH자유기、간자유기급초양음리자자유기균구유교강청제작용，반수억제농도분별위0.552、0.282화0.111 mg/mL。
The xylanase extraction with ultrasonic–assisted technologyon ferulic acidofcorn bran wasoptimized by response surface methodology with mathematical modelof four factors,which were ultrasonic temperature,ultrasonic time,enzyme dosage and reaction time,and the antioxidant capacityof ferulic acid was evaluated by free radical scavenging activity. The results showed that the optimal extraction yieldof ferulic acid fromcorn bran was 3.47 mg/g with ultrasonic extraction at 33℃ for 80 min and separated by 0.83% xylanase at 50℃ for 65 min. Underoptimumconditions,the ferulic acidofcorn bran has a high anti–lipid peroxidation with scavenging effecton DPPH radicals, hydroxyl radicals and superoxide anions,the inhibitoryconcentration 50% indexes were 0.552 mg/mL, 0.282 mg/mL and 0.111 mg/mL,respectively.