CAJ | 학술논문

该文通过观察不同时期豉曲的菌丝生长情况，测定霉菌菌落总数、酶活力的动态变化，确定最佳的前发酵生产工艺，并对最佳条件下不同时期豉曲的酶活进行API ZYM（酶谱鉴定）系统的半定量分析。结果表明：在培养温度15℃、接种量1%、含水量45%、培养时间64 h时最佳，此时菌丝生长旺盛，产生大量孢子，霉菌菌落总数为2.1×107 CFU/g，总蛋白酶活力为103.33 U/g，纤维素酶活力为1.87 U/g，且通过API ZYM系统检测出了碱性磷酸盐酶、白氨酸芳胺酶、酸性磷酸酶、萘酚–AS–BI–磷酸水解酶等酶。
해문통과관찰불동시기시곡적균사생장정황，측정매균균락총수、매활력적동태변화，학정최가적전발효생산공예，병대최가조건하불동시기시곡적매활진행API ZYM（매보감정）계통적반정량분석。결과표명：재배양온도15℃、접충량1%、함수량45%、배양시간64 h시최가，차시균사생장왕성，산생대량포자，매균균락총수위2.1×107 CFU/g，총단백매활력위103.33 U/g，섬유소매활력위1.87 U/g，차통과API ZYM계통검측출료감성린산염매、백안산방알매、산성린산매、내분–AS–BI–린산수해매등매。
Toobtain theoptimal process parametersof the pro–fermentation processing,the growthof mycelium,the total numberof moldcolony,proteinase activity andcellulase activity wereobserved and determined. Under theoptimal pro–fermentation processing,the API ZYM system was applicated to semi–quantiative analysisof enzymatic activity. The results show that:at 15℃,64 h,1% inoculum size and 45% moisturecontent,hyphagrew vigorously,a large numberof spores produced,the total numberof moldcolony was 2.1×107 CFU/g,total protease activity andcellulose activity reached 103.33 U/g and 1.87 U/g,respectively. Alkaline phosphatase,Leucine arylamidase,Acid phosphatase and Naphtol–AS–BI–phosphohydrilase were detected by API ZYM system.