检验医学与临床
檢驗醫學與臨床
검험의학여림상
Laboratory Medicine and Clinic
2015年
21期
3144-3146,3149
,共4页
梅序桥%吴阿阳%郑燕苹%陈宏浦%郑源海%杨惠聪%张佳林
梅序橋%吳阿暘%鄭燕蘋%陳宏浦%鄭源海%楊惠聰%張佳林
매서교%오아양%정연평%진굉포%정원해%양혜총%장가림
类风湿关节炎%流式细胞%T淋巴细胞亚群
類風濕關節炎%流式細胞%T淋巴細胞亞群
류풍습관절염%류식세포%T림파세포아군
rheumatoid arthritis%flow cytometry%T-lymphocytes subsets
目的：探讨类风湿关节炎（RA ）患者外周血T淋巴细胞亚群CD4＋／CD8＋的表达变化对疾病进展的关系。方法采用流式细胞术检测94例RA患者和22例健康对照者外周血T淋巴细胞亚群CD4＋／CD8＋。其中94例RA患者按RA疾病活动指数（DAS28）评分分为低值、中值、高值3组。结果与健康对照组比较，RA组外周血中CD3＋CD4＋ T淋巴细胞的表达率明显升高（ P＜0．05），其中CD3＋ CD4＋ T 淋巴细胞在DAS28低值组为（41．03±9．53）％，DAS28中值组为（42．16±7．08）％，DAS28高值组为（43．72±9．63）％；CD3＋ CD8＋ T 淋巴细胞表达率明显降低（P＜0．05），其中CD3＋ CD8＋ T 淋巴细胞在DAS28低值组为（21．33±6．67）％，DAS28中值组为（21．39±7．36）％，DAS28高值组为（21．28±6．60）％。结论 CD4＋／CD8＋比值与RA 患者疾病进程相关，表明CD4＋／CD8＋失衡在RA的发病中发挥了重要作用。
目的：探討類風濕關節炎（RA ）患者外週血T淋巴細胞亞群CD4＋／CD8＋的錶達變化對疾病進展的關繫。方法採用流式細胞術檢測94例RA患者和22例健康對照者外週血T淋巴細胞亞群CD4＋／CD8＋。其中94例RA患者按RA疾病活動指數（DAS28）評分分為低值、中值、高值3組。結果與健康對照組比較，RA組外週血中CD3＋CD4＋ T淋巴細胞的錶達率明顯升高（ P＜0．05），其中CD3＋ CD4＋ T 淋巴細胞在DAS28低值組為（41．03±9．53）％，DAS28中值組為（42．16±7．08）％，DAS28高值組為（43．72±9．63）％；CD3＋ CD8＋ T 淋巴細胞錶達率明顯降低（P＜0．05），其中CD3＋ CD8＋ T 淋巴細胞在DAS28低值組為（21．33±6．67）％，DAS28中值組為（21．39±7．36）％，DAS28高值組為（21．28±6．60）％。結論 CD4＋／CD8＋比值與RA 患者疾病進程相關，錶明CD4＋／CD8＋失衡在RA的髮病中髮揮瞭重要作用。
목적：탐토류풍습관절염（RA ）환자외주혈T림파세포아군CD4＋／CD8＋적표체변화대질병진전적관계。방법채용류식세포술검측94례RA환자화22례건강대조자외주혈T림파세포아군CD4＋／CD8＋。기중94례RA환자안RA질병활동지수（DAS28）평분분위저치、중치、고치3조。결과여건강대조조비교，RA조외주혈중CD3＋CD4＋ T림파세포적표체솔명현승고（ P＜0．05），기중CD3＋ CD4＋ T 림파세포재DAS28저치조위（41．03±9．53）％，DAS28중치조위（42．16±7．08）％，DAS28고치조위（43．72±9．63）％；CD3＋ CD8＋ T 림파세포표체솔명현강저（P＜0．05），기중CD3＋ CD8＋ T 림파세포재DAS28저치조위（21．33±6．67）％，DAS28중치조위（21．39±7．36）％，DAS28고치조위（21．28±6．60）％。결론 CD4＋／CD8＋비치여RA 환자질병진정상관，표명CD4＋／CD8＋실형재RA적발병중발휘료중요작용。
Objective To investigate the correlation between the change of peripheral blood T‐lymphocyte subsets CD4/CD8 with the disease progress in the patients with rheumatoid arthritis (RA) .Methods The peripheral blood T‐lymphocytes subsets CD4/CD8 in 94 patients with RA and 22 healthy controls were examined by flow cy‐tometry .94 cases of RA were divided into low value ,median value and high value groups by RA disease activity index (DAS28) scores .Results Compared with the healthy controls ,the expression rate of peripheral blood CD3+CD4+ T cells in the RA group was increased significantly (P<0 .05) ,in which CD3+CD4+ T cells were (41 .03 ± 9 .53)% in DAS28 low value group ,(42 .16 ± 7 .08)% in DAS28 median value group and (43 .72 ± 9 .63)% in DAS28 high value group .The expression rate of CD3+CD8+ T was significantly decreased (P<0 .05) ,in which CD3+CD8+ T cells were (21 .33 ± 6 .67)% in DAS28 low value group ,(21 .39 ± 7 .36)% in DAS28 median value group and (21 .28 ± 6 .60)%in DAS28 high value group .Conclusion CD4+ /CD8+ ratio is associated with RA progression ,indicating that the CD4+ /CD8+ imbalances plays an important role in the pathogenesis of RA .
