中国妇幼健康研究
中國婦幼健康研究
중국부유건강연구
Chinese Journal of Woman and Child Health Research
2015年
5期
920-921,924
,共3页
恒定型自然杀伤T细胞%自发性早产%免疫%作用机制
恆定型自然殺傷T細胞%自髮性早產%免疫%作用機製
항정형자연살상T세포%자발성조산%면역%작용궤제
invariant natural killer T-cells ( iNKT)%spontaneous preterm%immune%action mechanism
目的：探讨恒定型自然杀伤T细胞（ iNKT）在自发性早产发动中的作用。方法选择自发性早产孕妇40例（早产组）和正常孕妇40例（对照组）。采用流式细胞术检测蜕膜组织中iNKT细胞和自然杀伤（ NK）细胞的表达；采用RT-PCR法检测穿孔素（perforin）mRNA的表达。结果早产组蜕膜组织中iNKT细胞和NK细胞的百分比分别为（1．05±0．29）％和（24．21±9．32）％，均显著高于对照组iNKT细胞（0．48±0．17）％和NK细胞（14．97±6．05）％的百分比（t值分别为2．267和2．094，均P＜0．05）；经Spearman秩相关分析显示，早产组iNKT细胞和NK细胞呈明显的正相关（r＝0．634，P＜0．05）；RT-PCR结果显示，早产组穿孔素mRNA的表达也显著高于对照组（ t＝2．086，P＜0．05）。结论 iNKT细胞可能通过产生穿孔素并促进NK细胞活化，参与自发性早产的免疫发病机制。
目的：探討恆定型自然殺傷T細胞（ iNKT）在自髮性早產髮動中的作用。方法選擇自髮性早產孕婦40例（早產組）和正常孕婦40例（對照組）。採用流式細胞術檢測蛻膜組織中iNKT細胞和自然殺傷（ NK）細胞的錶達；採用RT-PCR法檢測穿孔素（perforin）mRNA的錶達。結果早產組蛻膜組織中iNKT細胞和NK細胞的百分比分彆為（1．05±0．29）％和（24．21±9．32）％，均顯著高于對照組iNKT細胞（0．48±0．17）％和NK細胞（14．97±6．05）％的百分比（t值分彆為2．267和2．094，均P＜0．05）；經Spearman秩相關分析顯示，早產組iNKT細胞和NK細胞呈明顯的正相關（r＝0．634，P＜0．05）；RT-PCR結果顯示，早產組穿孔素mRNA的錶達也顯著高于對照組（ t＝2．086，P＜0．05）。結論 iNKT細胞可能通過產生穿孔素併促進NK細胞活化，參與自髮性早產的免疫髮病機製。
목적：탐토항정형자연살상T세포（ iNKT）재자발성조산발동중적작용。방법선택자발성조산잉부40례（조산조）화정상잉부40례（대조조）。채용류식세포술검측세막조직중iNKT세포화자연살상（ NK）세포적표체；채용RT-PCR법검측천공소（perforin）mRNA적표체。결과조산조세막조직중iNKT세포화NK세포적백분비분별위（1．05±0．29）％화（24．21±9．32）％，균현저고우대조조iNKT세포（0．48±0．17）％화NK세포（14．97±6．05）％적백분비（t치분별위2．267화2．094，균P＜0．05）；경Spearman질상관분석현시，조산조iNKT세포화NK세포정명현적정상관（r＝0．634，P＜0．05）；RT-PCR결과현시，조산조천공소mRNA적표체야현저고우대조조（ t＝2．086，P＜0．05）。결론 iNKT세포가능통과산생천공소병촉진NK세포활화，삼여자발성조산적면역발병궤제。
Objective To investigate the role of invariant natural killer T-cells ( iNKT) cells in the launch of spontaneous preterm labor. Methods Totally 40 preterm pregnant women ( preterm group ) and 40 normal pregnant women ( control group ) were selected.Flow cytometry was used to detect iNKT cells and NK cells in decidual tissue, and RT-PCR was used to detect the expression of perforin mRNA. Results The percentages of iNKT cells and NK cells in decidual tissue in the preterm group were (1.05 ±0.29)% and (24.21 ± 9.32)%, respectively, which were significantly higher than those of iNKT cells (0.48 ±0.17)%and NK cells (14.97 ±6.05)%in the control group (t value was 2.267 and 2.094, respectively, both P<0.05).Spearman rank correlation analysis showed that iNKT cells was positively related with NK cells in the preterm group (r=0.634,P<0.05).RT-PCR results revealed that the expression of perforin mRNA in the preterm group was significantly higher than that in the control group (t=2.086, P<0.05).Conclusion INKT cells may take part in the immune pathogenesis of spontaneous preterm labor through the production of perforin and the promotion of NK cell activation.
