CAJ | 학술논문

目的：明确陕西省及周边地区成年女性宫颈人乳头瘤病毒（ HPV）感染基因亚型的分布及年龄特征。方法分析2009年11月至2015年4月在西安交通大学第一附属医院妇科门诊22306例宫颈样本21种HPV基因分型检测结果。 HPV分型检测采用凯普医用核酸分子快速导流杂交基因芯片技术。结果 HPV总感染率为28．84％。高危型HPV（16、18、31、33、35、39、45、51、52、53、56、58、59、66、68）感染率为26．26％，占HPV阳性标本的91．05％；低危型HPV（6、11、42、43、44、81）感染率为4．81％；高危／低危HPV混合感染率为2．22％。感染率最高的基因亚型为HPV16（9．31％），其他常见高危亚型依次为HPV52（5．08％）、HPV58（4．99％）、HPV18（2．09％）、HPV31（1．95％）、HPV39（1．86％）、HPV33（1．74％）；常见低危亚型为 HPV6（2．02％）、11（1．56％）、81（1．02％）。 HPV总体感染率和高危HPV亚型感染高峰年龄在30～34岁之间，以后随年龄增长逐渐降低，各年龄组间HPV检出率有显著性差异（χ2值为4．743～85．971，均P＜0．05）。结论陕西省及周边地区女性宫颈HPV处于较高的感染水平；其HPV感染基因型符合亚洲人群分布规律，但有一定区域性差异；随着年龄的增高，高危型HPV感染比例逐步缓慢增加。该结果对区域宫颈癌防治和疫苗制备具指导意义。
목적：명학합서성급주변지구성년녀성궁경인유두류병독（ HPV）감염기인아형적분포급년령특정。방법분석2009년11월지2015년4월재서안교통대학제일부속의원부과문진22306례궁경양본21충HPV기인분형검측결과。 HPV분형검측채용개보의용핵산분자쾌속도류잡교기인심편기술。결과 HPV총감염솔위28．84％。고위형HPV（16、18、31、33、35、39、45、51、52、53、56、58、59、66、68）감염솔위26．26％，점HPV양성표본적91．05％；저위형HPV（6、11、42、43、44、81）감염솔위4．81％；고위／저위HPV혼합감염솔위2．22％。감염솔최고적기인아형위HPV16（9．31％），기타상견고위아형의차위HPV52（5．08％）、HPV58（4．99％）、HPV18（2．09％）、HPV31（1．95％）、HPV39（1．86％）、HPV33（1．74％）；상견저위아형위 HPV6（2．02％）、11（1．56％）、81（1．02％）。 HPV총체감염솔화고위HPV아형감염고봉년령재30～34세지간，이후수년령증장축점강저，각년령조간HPV검출솔유현저성차이（χ2치위4．743～85．971，균P＜0．05）。결론합서성급주변지구녀성궁경HPV처우교고적감염수평；기HPV감염기인형부합아주인군분포규률，단유일정구역성차이；수착년령적증고，고위형HPV감염비례축보완만증가。해결과대구역궁경암방치화역묘제비구지도의의。
Objective To analyze HPV genotype distribution and age characteristics among women in Shaanxi Province and areas nearby. Methods A total of 22 306 cervical scraping samples were collected from the outpatients for cervical cancer screening in the First Affiliated Hospital of Xi’ an Jiaotong University during November 2009 to April 2015.Twenty-one HPV genotypes were examined by PCR-based hybridization gene chip assay.Results The overall HPV infection rate was 28.84%.The infection rate of high-risk HPV (16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68) was 26.26%, which occupied 91.05% of HPV positive samples.There were 4.81%cases being confirmed to have low-risk HPV (6, 11, 42, 43, 44, 81), and the combined infection rate of both high-risk and low-risk HPV was 2.22%.The most prevalent high-risk HPV was HPV16 (9.31%), followed by high-risk HPV 52 (5.08%), HPV 58 (4.99%), HPV 18 (2.09%), HPV 31 (1.95%), HPV 39 (1.86%), and HPV 33 (1.74%), respectively.The most prevalent low-risk HPV were HPV6 (2.02%), HPV 11 (1.56%), and HPV 81 (1.02%).The overall infection rate of HPV and infection rate of high-risk HPV reached peak among the women aged 30-34 years, and they declined with age increasing.The difference in HPV detection rate was significant among different age groups (χ2 ranged 4.743-85.971, P<0.05).Conclusion HPV infection rate is high in Shaanxi Province and surrounding areas.The infection genotype of HPV in Shaanxi area has similar spectrum to that in Asian regions but with slight difference.The infection rate of high-risk HPV increases gradually with age increasing.These data are helpful for the cervical cancer prevention and the vaccination program in this area.