CAJ | 학술논문

目的：探讨内源性肿瘤坏死因子α（ TNF-α）在小儿结肠息肉中的表达及临床意义。方法采集45例结肠息肉患儿（实验组）及36例正常结肠粘膜儿童（对照组）的血清，运用夹心法酶联免疫吸附试验（ ELISA）方法检测TNF-α在两组血清中的表达；免疫组化法检测TNF-α在两组患儿结肠组织中的表达，分析TNF-α的表达与结肠息肉的关系。结果 TNF-α在实验组血清中的表达高于对照组（t＝5．360，P＜0．05），在实验组结肠组织中表达高于对照组（χ2＝10．434，P＜0．05）；在实验组中，腺瘤性息肉中TNF-α的表达较幼年性息肉中无明显差异（P＞0．05）。结论 TNF-α在结肠息肉组织中的高表达，提示TNF-α在儿童结肠息肉的发生发展中可能起重要作用，有可能成为判断结肠息肉预后复发的标志物。
목적：탐토내원성종류배사인자α（ TNF-α）재소인결장식육중적표체급림상의의。방법채집45례결장식육환인（실험조）급36례정상결장점막인동（대조조）적혈청，운용협심법매련면역흡부시험（ ELISA）방법검측TNF-α재량조혈청중적표체；면역조화법검측TNF-α재량조환인결장조직중적표체，분석TNF-α적표체여결장식육적관계。결과 TNF-α재실험조혈청중적표체고우대조조（t＝5．360，P＜0．05），재실험조결장조직중표체고우대조조（χ2＝10．434，P＜0．05）；재실험조중，선류성식육중TNF-α적표체교유년성식육중무명현차이（P＞0．05）。결론 TNF-α재결장식육조직중적고표체，제시TNF-α재인동결장식육적발생발전중가능기중요작용，유가능성위판단결장식육예후복발적표지물。
Objective To investigate the expression and clinical significance of endogenous tumor necrosis factor alpha ( TNF-α) in children with colonic polyps.Methods Serum of 45 cases ( experimental group) with colonic polyps and 36 cases ( control group) with normal colonic mucosa was detected with enzyme-linked immunosorbent assay ( ELISA ) to explore the expression of TNF-α. Immunohistochemical method was used to detect the expression of TNF-αin colonic tissue.The relationship between expression of TNF-αand colonic polyps was analyzed.Results The expression of TNF-αin serum was higher in the experimental group than in the control group (t=5.360, P<0.05), so was in colonic tissue (χ2 =10.434,P<0.05).In the experimental group the expression of TNF-αwas not significantly different between adenomatous polyp and juvenile polyp (P>0.05).Conclusion High expression of TNF-αin colon polyps suggests that TNF-αmay play an important role in the development and progression of colon polyps in children.It may be a marker for judging the prognosis of colon polyps.