临床与病理杂志
臨床與病理雜誌
림상여병리잡지
International Journal of Pathology and Clinical Medicine
2015年
10期
1827-1831
,共5页
手足口病%淋巴细胞亚群%流式细胞术%脑炎%儿童
手足口病%淋巴細胞亞群%流式細胞術%腦炎%兒童
수족구병%림파세포아군%류식세포술%뇌염%인동
hand,foot and mouth disease%lymphocyte subsets%lfow cytometry%cerebritis%children
目的：研究手足口病(hand foot and mouth disease，HFMD)并发脑炎患儿外周血淋巴细胞亚群的变化，以探讨其免疫功能改变与疾病的关系。方法：收集2014年12月至2015年5月徐州市儿童医院收治的57例手足口病患儿的临床资料，根据病情严重程度分为手足口病并发脑炎组34例和手足口病未并发脑炎组23例，以20例同期进行腹股沟疝外科手术的儿童为对照组。应用流式细胞仪检测外周抗凝全血的淋巴细胞亚群：T淋巴细胞(CD3+CD19?)、辅助T细胞Th(CD3+CD4+)、抑制T细胞Ts(CD3+CD8+)、B淋巴细胞(CD3?CD19+)和NK细胞(CD3?CD56+/CD16+)的相对计数。结果：手足口病并发脑炎组患儿外周血T淋巴细胞(CD3+CD19?)、辅助T细胞Th(CD3+CD4+)、抑制T细胞Ts(CD3+CD8+)的百分率下降，明显低于手足口病未并发脑炎组及对照组(P<0.05)；B淋巴细胞(CD3?CD19+)的百分率较其它2组明显增高(P<0.05)；但手足口病未并发脑炎组与对照组之间辅助T细胞Th(CD3+CD4+)、抑制T细胞Ts(CD3+CD8+)、B淋巴细胞(CD3?CD19+)的差异无统计学意义(P>0.05)。手足口病并发脑炎组及手足口病未并发脑炎组NK细胞(CD3?CD56+/CD16+)百分率均高于对照组(P<0.05)；但二者之间差异无显著性统计学意义(P>0.05)。结论：手足口病患儿特别是合并有脑炎的重症手足口病患儿存在明显的免疫功能低下及免疫调节紊乱。
目的：研究手足口病(hand foot and mouth disease，HFMD)併髮腦炎患兒外週血淋巴細胞亞群的變化，以探討其免疫功能改變與疾病的關繫。方法：收集2014年12月至2015年5月徐州市兒童醫院收治的57例手足口病患兒的臨床資料，根據病情嚴重程度分為手足口病併髮腦炎組34例和手足口病未併髮腦炎組23例，以20例同期進行腹股溝疝外科手術的兒童為對照組。應用流式細胞儀檢測外週抗凝全血的淋巴細胞亞群：T淋巴細胞(CD3+CD19?)、輔助T細胞Th(CD3+CD4+)、抑製T細胞Ts(CD3+CD8+)、B淋巴細胞(CD3?CD19+)和NK細胞(CD3?CD56+/CD16+)的相對計數。結果：手足口病併髮腦炎組患兒外週血T淋巴細胞(CD3+CD19?)、輔助T細胞Th(CD3+CD4+)、抑製T細胞Ts(CD3+CD8+)的百分率下降，明顯低于手足口病未併髮腦炎組及對照組(P<0.05)；B淋巴細胞(CD3?CD19+)的百分率較其它2組明顯增高(P<0.05)；但手足口病未併髮腦炎組與對照組之間輔助T細胞Th(CD3+CD4+)、抑製T細胞Ts(CD3+CD8+)、B淋巴細胞(CD3?CD19+)的差異無統計學意義(P>0.05)。手足口病併髮腦炎組及手足口病未併髮腦炎組NK細胞(CD3?CD56+/CD16+)百分率均高于對照組(P<0.05)；但二者之間差異無顯著性統計學意義(P>0.05)。結論：手足口病患兒特彆是閤併有腦炎的重癥手足口病患兒存在明顯的免疫功能低下及免疫調節紊亂。
목적：연구수족구병(hand foot and mouth disease，HFMD)병발뇌염환인외주혈림파세포아군적변화，이탐토기면역공능개변여질병적관계。방법：수집2014년12월지2015년5월서주시인동의원수치적57례수족구병환인적림상자료，근거병정엄중정도분위수족구병병발뇌염조34례화수족구병미병발뇌염조23례，이20례동기진행복고구산외과수술적인동위대조조。응용류식세포의검측외주항응전혈적림파세포아군：T림파세포(CD3+CD19?)、보조T세포Th(CD3+CD4+)、억제T세포Ts(CD3+CD8+)、B림파세포(CD3?CD19+)화NK세포(CD3?CD56+/CD16+)적상대계수。결과：수족구병병발뇌염조환인외주혈T림파세포(CD3+CD19?)、보조T세포Th(CD3+CD4+)、억제T세포Ts(CD3+CD8+)적백분솔하강，명현저우수족구병미병발뇌염조급대조조(P<0.05)；B림파세포(CD3?CD19+)적백분솔교기타2조명현증고(P<0.05)；단수족구병미병발뇌염조여대조조지간보조T세포Th(CD3+CD4+)、억제T세포Ts(CD3+CD8+)、B림파세포(CD3?CD19+)적차이무통계학의의(P>0.05)。수족구병병발뇌염조급수족구병미병발뇌염조NK세포(CD3?CD56+/CD16+)백분솔균고우대조조(P<0.05)；단이자지간차이무현저성통계학의의(P>0.05)。결론：수족구병환인특별시합병유뇌염적중증수족구병환인존재명현적면역공능저하급면역조절문란。
Objective: To discuss the variation of peripheral blood lymphocyte subsets and the relationship between immunity and severity degree of hand, foot and mouth disease (HFMD) with complication of cerebritis in Children. Methods: Clinical data of 57 HFMD kids was collected from December 2014 to May 2015. 57 cases with HFMD were divided into two groups, HFMD with cerebritis group (34 cases with complication of cerebritis) and uncomplicated HFMD group (23 cases without complication). Meanwhile, 20 kids ready for inguinal hernia surgery as control.The percentage of lymphocyte subsets, including CD3+CD19? cell (T), CD3+CD4+ cell (Th), CD3+CD8+ cell (Ts), CD3?CD19+ cell (B), CD3?CD56+/CD16+ cell (NK) was determined by flow cytometry (FCM). Results: Compared with uncomplicated HFMD group and control group, the HFMD with cerebritis group displayed signiifcant decreases in their peripheral CD3+CD19? cell (T), CD3+CD4+ cell (hT) and CD3+CD8+ cell (Ts) subsets (P<0.05), and had a substantial increase in their peripheral CD3?CD19+ cell (B) subset (P<0.05). But no signiifcant difference between uncomplicated HFMD group and control group in the CD3+CD4+ cell (hT), CD3+CD8+ cell (Ts) and CD3?CD19+ cell (B) subsets. Compared with control group, the HFMD with cerebritis group and uncomplicated HFMD group showed an obvious increase in percentage of CD3?CD56+/CD16+ cell (NK) (P<0.05), but no signiifcant difference between the two groups (P>0.05).Conclusion:hTere are functional disorders in cellular immunity in HFMD children. hTe serious group with complication of cerebritis showed more serious disorder of immune function.
