CAJ | 학술논문

目的：探究阿司匹林在调控子宫内膜容受性分子HOXA10表达及胚胎黏附的作用及机制。方法：不同浓度的阿司匹林刺激Ishikawa细胞，相应时间点收取细胞总RNA和蛋白；特异性干扰HOXA10表达的siRNA转染入Ishikawa细胞。分别利用Real-time PCR和Western blot检测HOXA10的mRNA和蛋白水平。利用BeWo细胞球黏附于Ishikawa细胞的模型检测阿司匹林在体外对胚胎黏附的影响。结果：经不同浓度的阿司匹林刺激后，相比于DMSO对照组，Ishikawa细胞中HOXA10的mRNA和蛋白水平都升高，在10?5 mol/L的阿司匹林处理组升高的最明显(P<0.01)。同时，阿司匹林促进BeWo细胞球黏附于Ishikawa细胞上，然而当特异性siRNA干扰HOXA10表达后，阿司匹林对胚胎黏附的促进作用被抑制。结论：阿司匹林可能通过上调子宫内膜容受性分子HOXA10的表达促进胚胎的黏附。
목적：탐구아사필림재조공자궁내막용수성분자HOXA10표체급배태점부적작용급궤제。방법：불동농도적아사필림자격Ishikawa세포，상응시간점수취세포총RNA화단백；특이성간우HOXA10표체적siRNA전염입Ishikawa세포。분별이용Real-time PCR화Western blot검측HOXA10적mRNA화단백수평。이용BeWo세포구점부우Ishikawa세포적모형검측아사필림재체외대배태점부적영향。결과：경불동농도적아사필림자격후，상비우DMSO대조조，Ishikawa세포중HOXA10적mRNA화단백수평도승고，재10?5 mol/L적아사필림처리조승고적최명현(P<0.01)。동시，아사필림촉진BeWo세포구점부우Ishikawa세포상，연이당특이성siRNA간우HOXA10표체후，아사필림대배태점부적촉진작용피억제。결론：아사필림가능통과상조자궁내막용수성분자HOXA10적표체촉진배태적점부。
Objective: To explore the role and mechanism of aspirin in regulating the expression of endometrial receptivity marker gene HOXA10 and embryo implantation.Methods:In this study, Ishikawa cells were treated with different doses of aspirin for indicated time and transfected with siRNAs against HOXA10. hTe expression of HOXA10 mRNA and protein in Ishikawa cells were determined by Real-time PCR and Western blot. Furthermore, the effect of aspirin on embryo implantationin vitro was determined by anin vitro model of BeWo-Ishikawa cell interaction. Results:Compared with the control group treated with DMSO, the expression of HOXA10 mRNA and protein were up-regulated in Ishikawa cells treated with different doses of aspirin. And, the mRNA and protein levels of HOXA10 were highest in Ishikawa cells treated with aspirin at the dose of 10?5 mol/L (P<0.01). Furthermore,aspirin promoted BeWo spheroids attachment to Ishikawa cells, however, the effect of aspirin on BeWo spheroids attachment was inhibited by HOXA10-specific siRNAs in Ishikawa cells.Conclusion: Aspirin may promote embryo implantation through up-regulation of endometrial receptivity marker gene HOXA10.