中南大学学报（英文版）
中南大學學報（英文版）
중남대학학보（영문판）
Journal of Central South University
2015年
11期
4162-4167
,共6页
β-mannanase%Pichia pastoris%high-production strains%hydrolysis hole screening method%micro-plate screening method
The yeastPichia pastoris(P. pastoris) has been used for the expression of heterologous proteins with the significant success. However, it is time-consuming to screen the high expression level of the recombinantP. pastorisdirectly. Thus, forβ-mannanase production, developing the accurate, rapid and inexpensive screening method to substitute random screening is certainly required. A simple method based on the size of hydrolysis hole was described here, but this method was not very accurate that could only be used in preliminary screening. To further improve the accuracy, a micro-plate screening method is established, which appears to be more accurate and effective. The efficiency of this screening method is about 10 times higher than that of the general screening strategy of cultivation in shaking flasks. Two methods presented here can also be used for screening of recombinantPichia strains with high-level expression of other heterologous protein after modification.
