CAJ | 학술논문

目的：探讨铁镍软磁合金应用于临床的生物安全性。方法将5种材料（纯钛、铁镍合金、电镀Cr6+的铁镍合金、钴铬合金、PVC）制成2 cm ×2 cm ×0.2 cm的方块，消毒灭菌后制取材料浸提液。取5块无菌96孔板，每孔加入100μl浓度为6×104个/ml对数生长期L?929细胞悬液，贴壁生长24 h后置换各组材料浸提液。培养1、2、3、4、5 d后各取出1块板，于倒置显微镜下观察各组细胞形态和贴壁情况，然后向每孔加入10μl CCK?8试剂，避光培养1.5 h后用酶标仪测定各孔吸光度（A）值。利用各组之间A值计算细胞相对增殖率（RGR），并对各组实验材料细胞毒性进行分级评价。结果在倒置显微镜下，除PVC出现大量细胞溶解、固缩外，其余各组细胞均形态正常、贴壁生长良好。根据各组材料细胞RGR进行毒性分级可知，除外PVC组为3级，其余各实验组材料毒性级别均为0~1级，可以应用于人体。结论铁镍合金原样和电镀Cr6+后的细胞毒性均在安全范围。
목적：탐토철얼연자합금응용우림상적생물안전성。방법장5충재료（순태、철얼합금、전도Cr6+적철얼합금、고락합금、PVC）제성2 cm ×2 cm ×0.2 cm적방괴，소독멸균후제취재료침제액。취5괴무균96공판，매공가입100μl농도위6×104개/ml대수생장기L?929세포현액，첩벽생장24 h후치환각조재료침제액。배양1、2、3、4、5 d후각취출1괴판，우도치현미경하관찰각조세포형태화첩벽정황，연후향매공가입10μl CCK?8시제，피광배양1.5 h후용매표의측정각공흡광도（A）치。이용각조지간A치계산세포상대증식솔（RGR），병대각조실험재료세포독성진행분급평개。결과재도치현미경하，제PVC출현대량세포용해、고축외，기여각조세포균형태정상、첩벽생장량호。근거각조재료세포RGR진행독성분급가지，제외PVC조위3급，기여각실험조재료독성급별균위0~1급，가이응용우인체。결론철얼합금원양화전도Cr6+후적세포독성균재안전범위。
Objective To research the cytotoxicity of Fe?Ni soft magnetic alloy in order to provide a biological basis for the clinical application. Methods Five groups of materials(Pure Ti,Fe?Ni alloy, Fe?Ni plated Cr6+,Co?Cr alloy,and PVC)were cut into 2 cm × 2 cm × 0.2 cm blocks and prepared for the extract after sterilization. L?929 cells were incubated in five 96?well culture plates at a concentration of 6 × 103 per well for 24 h to allow the attachment. The culture medium was replaced with 100μl exaction per well. After 1,2,3,4 and 5 d culturing,the cells of each groups were observed under microscope. 10μl CCK?8 solution was added to each well and cultured for 1.5 h,the absorbance values were obtained with a spectrophotometer. The cytotoxicity of the materials was evaluated by calculating the relative growth rate (RGR). Results The shape of L?929 cells of each group was normal besides the PVC group under the microscope. The cytotoxicity of PVC was grade 3,and all the other materials were grade 0~1 based on the RGR. All of the experimental materials were safe for clinical application except PVC. Conclusion Both Fe?Ni alloy and Fe?Ni plated Cr6+have good biocompatibility.