江苏农业学报
江囌農業學報
강소농업학보
Jiangsu Journal of Agricultural Sciences
2015年
5期
975-983
,共9页
赵君%刘剑光%吴巧娟%赵亮%许剑文%肖松华
趙君%劉劍光%吳巧娟%趙亮%許劍文%肖鬆華
조군%류검광%오교연%조량%허검문%초송화
棉花%含油量%遗传多样性%关联分析
棉花%含油量%遺傳多樣性%關聯分析
면화%함유량%유전다양성%관련분석
cotton%oil content%genetic diversi-ty%association analysis
棉花是重要的经济作物,提高棉子的含油量具有重要的经济效益和社会意义。本研究以24份高油和低酚棉花品种(系)为材料,测定种仁的含油量及脂肪酸组分。结果显示,近期育成的棉花品种(系)种仁含油量显著低于高油材料的含油量,脂肪酸组分在不同品种(系)间存在显著差异,但与含油量之间没有显著的相关性。利用均匀分布于棉花26条染色体上的370对SSR标记对32个棉花品种(系)进行遗传多样性分析。共获得190个多态性位点,检测到609个等位变异,各位点检测到的等位变异数变幅为2~9个,平均为3.09个,多态信息含量(PIC)变幅为0.07~0.81,平均为0.40。聚类分析将32份材料分为5个类群,其中12份高含油量种质材料划分成3个类群,剩余的20份种质材料划分成2个类群。关联分析检测到与种仁含油量性状显著关联的位点11个,分布在9条染色体上,用两种方法计算结果显示其解释表型变异为0.0436~0.1449。
棉花是重要的經濟作物,提高棉子的含油量具有重要的經濟效益和社會意義。本研究以24份高油和低酚棉花品種(繫)為材料,測定種仁的含油量及脂肪痠組分。結果顯示,近期育成的棉花品種(繫)種仁含油量顯著低于高油材料的含油量,脂肪痠組分在不同品種(繫)間存在顯著差異,但與含油量之間沒有顯著的相關性。利用均勻分佈于棉花26條染色體上的370對SSR標記對32箇棉花品種(繫)進行遺傳多樣性分析。共穫得190箇多態性位點,檢測到609箇等位變異,各位點檢測到的等位變異數變幅為2~9箇,平均為3.09箇,多態信息含量(PIC)變幅為0.07~0.81,平均為0.40。聚類分析將32份材料分為5箇類群,其中12份高含油量種質材料劃分成3箇類群,剩餘的20份種質材料劃分成2箇類群。關聯分析檢測到與種仁含油量性狀顯著關聯的位點11箇,分佈在9條染色體上,用兩種方法計算結果顯示其解釋錶型變異為0.0436~0.1449。
면화시중요적경제작물,제고면자적함유량구유중요적경제효익화사회의의。본연구이24빈고유화저분면화품충(계)위재료,측정충인적함유량급지방산조분。결과현시,근기육성적면화품충(계)충인함유량현저저우고유재료적함유량,지방산조분재불동품충(계)간존재현저차이,단여함유량지간몰유현저적상관성。이용균균분포우면화26조염색체상적370대SSR표기대32개면화품충(계)진행유전다양성분석。공획득190개다태성위점,검측도609개등위변이,각위점검측도적등위변이수변폭위2~9개,평균위3.09개,다태신식함량(PIC)변폭위0.07~0.81,평균위0.40。취류분석장32빈재료분위5개류군,기중12빈고함유량충질재료화분성3개류군,잉여적20빈충질재료화분성2개류군。관련분석검측도여충인함유량성상현저관련적위점11개,분포재9조염색체상,용량충방법계산결과현시기해석표형변이위0.0436~0.1449。
Cotton is an important economic crop. It has great economic and social benefits to improve oil content of cotton seeds. In this study, seed oil content and fatty acid composition of 24 cotton varieties were measured. The results showed that the oil contents of recently released cotton varieties were significantly lower than high oil materials, and fatty acid composition varied among different varieties. There was no correlation between fatty acid and oil content. 370 pairs of cotton SSR primers distributed on 26 chromosomes of cotton were used for the diversity analysis of 32 cotton varieties. 190 polymor-phic loci and 609 alleles were detected. The allele number was 2-9 for these primers, with the average of 3. 09, and polymor-phic information content was about 0. 07-0. 81, with the average of 0. 40. 32 varieties were clustered into five sub-branches, among which, all high oil materials were grouped in three sub-branches and the remaining 20 varieties in other two. By asso-ciation analysis, 11 loci related to oil content traits were detected on nine chromosomes of cotton, which explained 0. 043 6-0. 144 9 of the phenotypic variation.