CAJ | 학술논문

根据转基因玉米 MIR604外源载体序列，设计引物和探针，并优化反应体系和热循环条件，建立了MIR604结构特异片断实时荧光定量PCR检测方法。采用该方法对6种非转基因作物、MIR604及其他非目标转基因作物进行检测，除MIR604外，其余样品均未检测到MIR604分子片段的扩增信号；对含量为1%的MIR604标准品进行定量检测，测试样品平均含量为1.05%；灵敏度测试结果显示该方法能检测到仅5个拷贝的MIR604分子片段。因此，本研究建立的方法具有较高的特异性、准确度及灵敏度，能为中国转基因生物安全监管提供技术支持。
근거전기인옥미 MIR604외원재체서렬，설계인물화탐침，병우화반응체계화열순배조건，건립료MIR604결구특이편단실시형광정량PCR검측방법。채용해방법대6충비전기인작물、MIR604급기타비목표전기인작물진행검측，제MIR604외，기여양품균미검측도MIR604분자편단적확증신호；대함량위1%적MIR604표준품진행정량검측，측시양품평균함량위1.05%；령민도측시결과현시해방법능검측도부5개고패적MIR604분자편단。인차，본연구건립적방법구유교고적특이성、준학도급령민도，능위중국전기인생물안전감관제공기술지지。
A structure-specific quantitative PCR technique for the detection of genetically modified maize MIR604 was developed in this study to provide supports for genetically modified organism safety supervision in China by optimizing reaction system and thermal cycling condition. Six kinds of non-genetically modified crops, MIR604 and other non-target transgenic crops were detected. The fluorescent signal was only detected in MIR604. The measured value of 1% MIR604 was 1. 05%. The sensitivity experiment revealed the method could detect only 5 copies of MIR604 molecular fragments. In conclusion, the structure-specific quantitative PCR detection method for genetically modified maize MIR604 is of high spe-cificity, accuracy and sensitivity.