CAJ | 학술논문

为了研究转基因小麦N12-1对其根际土壤中禾谷多黏菌( Polymyxa graminis)数量的影响,建立了基于实时荧光定量PCR( Quantitative Real-Time PCR)方法检测多黏菌数量的体系,并将该体系用于转基因小麦N12-1及其受体扬麦158不同生长期根际土壤中禾谷多黏菌数量的检测。结果显示,标准曲线的扩增效率为95%,相关系数为0.999,熔解曲线呈现单一峰,符合荧光定量扩增的各项指标；在各个生长期,转基因小麦N12-1与其受体扬麦158根际土壤中的禾谷多黏菌数量没有显著差异。由此推测,转基因小麦N12-1的种植对其根际土壤中禾谷多黏菌的数量没有显著影响。
위료연구전기인소맥N12-1대기근제토양중화곡다점균( Polymyxa graminis)수량적영향,건립료기우실시형광정량PCR( Quantitative Real-Time PCR)방법검측다점균수량적체계,병장해체계용우전기인소맥N12-1급기수체양맥158불동생장기근제토양중화곡다점균수량적검측。결과현시,표준곡선적확증효솔위95%,상관계수위0.999,용해곡선정현단일봉,부합형광정량확증적각항지표；재각개생장기,전기인소맥N12-1여기수체양맥158근제토양중적화곡다점균수량몰유현저차이。유차추측,전기인소맥N12-1적충식대기근제토양중화곡다점균적수량몰유현저영향。
A quantitative real-time PCR system for the detection of the number of Polymyxa graminis was established in this study. In the system, the standard curve showed a linear correlation between the input DNA and the CT values, with PCR efficiency of 95% and the correlation coefficient of 0. 999, and the melting curve presented a single peak, indicative of the feasibility of the system. The quantity of P. graminis in the rhizosphere soil of transgenic wheat N12-1 and its paren-tal material Yangmai158 during growth stages was detected using the system. No significant difference was detected in the quantity of P. graminis between transgenic wheat N12-1 and Yangmai158. The results suggested that the planting of trans-genic wheat N12-1 had few adverse effects on the quantity of P. graminis in rhizosphere soil.