CAJ | 학술논문

为了从分子水平上揭示北京白羽鹌鹑群体的遗传结构,为北京白羽鹌鹑遗传资源的评价、保护和利用提供新的依据,采用 PCR 扩增、聚丙烯酰胺凝胶电泳等方法对北京白羽鹌鹑群体9个微卫星标记( GUJ0023、GUJ0028、GUJ0029、GUJ0057、GUJ0059、GUJ0063、GUJ0077、GUJ0083、GUJ0097)的遗传多样性进行分析。结果表明,9个微卫星标记共检测出43个等位基因,平均每个标记检测到4.7778个等位基因。平均杂合度为0.7046,平均多态信息含量(PIC)为0.6587,平均有效等位基因数为3.6135。除GUJ0063(PIC=0.4639)在北京白羽鹌鹑群体中为中度多态性标记外,其余8个微卫星基因座均为高度多态性标记,可以作为北京白羽鹌鹑群体遗传多样性分析的有效遗传标记。微卫星基因座GUJ0023和GUJ0029在白羽鹌鹑群体中的χ2值小于χ20.01,符合哈迪-温伯格定律,其余微卫星基因座均偏离哈迪-温伯格平衡定律,这种遗传不平衡可能与近年来人们对北京白羽鹌鹑进行小群体保种有关。
위료종분자수평상게시북경백우암순군체적유전결구,위북경백우암순유전자원적평개、보호화이용제공신적의거,채용 PCR 확증、취병희선알응효전영등방법대북경백우암순군체9개미위성표기( GUJ0023、GUJ0028、GUJ0029、GUJ0057、GUJ0059、GUJ0063、GUJ0077、GUJ0083、GUJ0097)적유전다양성진행분석。결과표명,9개미위성표기공검측출43개등위기인,평균매개표기검측도4.7778개등위기인。평균잡합도위0.7046,평균다태신식함량(PIC)위0.6587,평균유효등위기인수위3.6135。제GUJ0063(PIC=0.4639)재북경백우암순군체중위중도다태성표기외,기여8개미위성기인좌균위고도다태성표기,가이작위북경백우암순군체유전다양성분석적유효유전표기。미위성기인좌GUJ0023화GUJ0029재백우암순군체중적χ2치소우χ20.01,부합합적-온백격정률,기여미위성기인좌균편리합적-온백격평형정률,저충유전불평형가능여근년래인문대북경백우암순진행소군체보충유관。
To reveal the genetic structure of Beijing white-feather quail at molecular level and to provide new evi-dence for the evaluation, protection and utilization of genetic resources of Beijing white-feather quail, the genetic diversity of Beijing white-feather quail population was analyzed with 9 microsatellite markers using PCR amplification, polyacrylam-ide gel electrophoresis. Totally 43 alleles were detected in 9 microsatellite markers GUJ0023, GUJ0028, GUJ0029, GUJ0057, GUJ0059, GUJ0063, GUJ0077, GUJ0083, GUJ0097, 4. 777 8 for each marker on average. The average het-erozygosity is 0. 704 6, the average polymorphism information content (PIC) is 0. 658 7 and the average effective allele is 3.613 5. Except for marker GUJ0063 (PIC=0.463 9) which was moderately polymorphic, the other 8 microsatellite markers showed high polymorphism which could serve as the effective genetic markers to analyze the genetic diversity of Beijing white-feather quail. Only GUJ0023 and GUJ0029 achieved the genetic distribution satisfying Hardy-Weinberg law in the white-feather quail population, while the rest markers significantly deviated from the Hardy-Weinberg balance ( P<0. 01) . The genetic disequilibrium might be associated with the small group conservation of Beijing white-feather quail in recent years.