CAJ | 학술논문

为探讨绵羊MHC-DQB1第2外显子基因多态性与乳房炎的抗性,采用PCR-SSCP方法对200只甘肃高山细毛羊和212只小尾寒羊MHC-DQB1第2外显子基因多态性进行了分析。结果表明,甘肃高山细毛羊和小尾寒羊的MHC-DQB1基因第2外显子均存在丰富多态性,2个绵羊品种共检测出14个等位基因,卡方适合性检验结果显示2个绵羊品种的SSCP带型均未达到哈德温伯格平衡。甘肃高山细毛羊中等位基因B (P<0.01,RR=0.158)与乳房炎抗性有较强相关性,等位基因F(P<0.01,RR=3.513)和L (P<0.01,RR=10.197)与乳房炎易感性具有较强相关性；小尾寒羊等位基因A(0.01<P<0.05,RR=0.878)与乳房炎抗性有相关性,等位基因E (P<0.01,RR=3.333)和M (P<0.01,RR=8.000)与乳房炎易感性具有较强相关性。表明2个绵羊品种的DQB1第2外显子存在丰富的多态性,甘肃高山细毛羊的等位基因B、F、L和小尾寒羊的等位基因A、E、M与乳房炎抗性和易感性相关。
위탐토면양MHC-DQB1제2외현자기인다태성여유방염적항성,채용PCR-SSCP방법대200지감숙고산세모양화212지소미한양MHC-DQB1제2외현자기인다태성진행료분석。결과표명,감숙고산세모양화소미한양적MHC-DQB1기인제2외현자균존재봉부다태성,2개면양품충공검측출14개등위기인,잡방괄합성검험결과현시2개면양품충적SSCP대형균미체도합덕온백격평형。감숙고산세모양중등위기인B (P<0.01,RR=0.158)여유방염항성유교강상관성,등위기인F(P<0.01,RR=3.513)화L (P<0.01,RR=10.197)여유방염역감성구유교강상관성；소미한양등위기인A(0.01<P<0.05,RR=0.878)여유방염항성유상관성,등위기인E (P<0.01,RR=3.333)화M (P<0.01,RR=8.000)여유방염역감성구유교강상관성。표명2개면양품충적DQB1제2외현자존재봉부적다태성,감숙고산세모양적등위기인B、F、L화소미한양적등위기인A、E、M여유방염항성화역감성상관。
To investigate the correlation between polymorphism of ovine major histocompatibility complexⅡDQB1 (MHC-DQB1)and mastitis, the DQB1 gene exon 2 in 200 Gansu alpine fine-wool sheep and 212 small tail Han sheep were amplified by PCR and characterized by single-strand conformation polymorphism(SSCP) technique. Abundant polymor-phism was detected in the exon 2 of MHC-DQB1. Fourteen alleles were found in the DQB1 gene exon 2. Results ofχ2 test showed that patterns of MHC-DQB1 were significantly deviant from Hardy-Weinberg equilibrium (P<0. 01). Al-lele B (P<0. 01,RR=0. 158) was correlated with mastitis resistance in Gansu alpine fine-wool sheep, while alleles F (P<0. 01,RR=3. 513) and L(P<0. 01,RR=10. 197) were correlated with mastitis susceptibility. Allele A (0. 01<P<0. 05,RR=0. 878), had strong correlation with mastitis resistance in small tail Han sheep, while alleles E (P<0. 01,RR=3. 333)and M (P<0. 01,RR=8. 000) had strong correlations with mastitis susceptibility. In sum, alleles B,F and L in Gansu alpine fine-wool sheep and alleles A, E and M in small tail Han sheep were relevant to susceptibility and re-sistance of mastitis.