CAJ | 학술논문

目的：研究松针层孔菌碱性多糖对单核细胞活性氧、凋亡和增殖的影响。方法自干燥松针层孔菌分离中等分子量松针层孔菌碱溶性多糖（MASPP）。体外培养单核系THP-1，采用不同浓度的MASPP（0、50、100、200 mg／L）诱导细胞24 h，流式细胞术检测活性氧和凋亡。不同浓度MASPP处理细胞72 h，WST-1还原法检测细胞增殖。结果诱导24 h， MASPP 以浓度依赖方式导致细胞内活性氧减少。对照组和200 mg／L MASPP组凋亡细胞百分率分别为4．32％和12．37％。 MASPP 处理72 h对细胞增殖有抑制作用，200 mg／L MASPP组的细胞增殖抑制率为（34．3±3．8）％。结论 MASPP可降低单核细胞活性氧、抑制细胞增殖并诱导细胞凋亡。
목적：연구송침층공균감성다당대단핵세포활성양、조망화증식적영향。방법자간조송침층공균분리중등분자량송침층공균감용성다당（MASPP）。체외배양단핵계THP-1，채용불동농도적MASPP（0、50、100、200 mg／L）유도세포24 h，류식세포술검측활성양화조망。불동농도MASPP처리세포72 h，WST-1환원법검측세포증식。결과유도24 h， MASPP 이농도의뢰방식도치세포내활성양감소。대조조화200 mg／L MASPP조조망세포백분솔분별위4．32％화12．37％。 MASPP 처리72 h대세포증식유억제작용，200 mg／L MASPP조적세포증식억제솔위（34．3±3．8）％。결론 MASPP가강저단핵세포활성양、억제세포증식병유도세포조망。
Objective To study reactive oxygen species (ROS),apoptosis and proliferation of monocyte affected by alkai-soluble Phellinus polysaccharide .Methods Middle molecular weight alkai-soluble Phellinus polysaccharide ( MASPP) was obtained from dried sporocarp of Phellinus pini.Monocyte THP-1 cells were cultured with different con-centration of MASPP (0 mg/L,50 mg/L,100 mg/L and 200 mg/L) for 24 hours.ROS and cell apoptosis were detec-ted by flow cytometry .Cell proliferation was measured by WST-1 reduction assay after cells were treated by MASPP for 72 hours.Results MASPP reduced intracellulor ROS dose-dependently .The percentage of apoptosis cells of control group and 200 mg/L MASPP treated for 24 hours were 4 .32%and 12 .37%respectively .The cells proliferation could be inhibited by MASPP for 72 hours,and the inhibition rate of 200 mg/L MASPP was (34.3 ±3.8)%.Conclusion MASPP could reduce ROS ,induce apoptosis and inhibit cell proliferation in monocytes .