CAJ | 학술논문

目的：探讨五味子乙素（ Sch B ）对人肝癌HepG2细胞的抑制作用，并研究其可能机制。方法10、25、50、75、100μmol／L Sch B作用于HepG2细胞，通过MTT法筛选低毒性的Sch B浓度，梯度浓度Sch B作用HepG2细胞48 h后，倒置显微镜下观察细胞形态；MTT法检测细胞活性；Western blot法检测Sch B作用HepG2细胞48 h后不同浓度组热休克蛋白-70（ Hsp-70）的表达情况；流式细胞仪检测细胞凋亡及周期改变。结果Sch B能够抑制HepG2细胞的生长，并呈浓度依赖性；流式细胞术结果显示10、25μmol／L Sch B组细胞在Sub-G1期明显高于阴性对照组（P＜0．05）。结论 Sch B促凋亡机制可能与下调Hsp-70蛋白的表达有关；如做免疫调节剂与其他抗癌药物联合使用最佳给药浓度为10μmol／L。
목적：탐토오미자을소（ Sch B ）대인간암HepG2세포적억제작용，병연구기가능궤제。방법10、25、50、75、100μmol／L Sch B작용우HepG2세포，통과MTT법사선저독성적Sch B농도，제도농도Sch B작용HepG2세포48 h후，도치현미경하관찰세포형태；MTT법검측세포활성；Western blot법검측Sch B작용HepG2세포48 h후불동농도조열휴극단백-70（ Hsp-70）적표체정황；류식세포의검측세포조망급주기개변。결과Sch B능구억제HepG2세포적생장，병정농도의뢰성；류식세포술결과현시10、25μmol／L Sch B조세포재Sub-G1기명현고우음성대조조（P＜0．05）。결론 Sch B촉조망궤제가능여하조Hsp-70단백적표체유관；여주면역조절제여기타항암약물연합사용최가급약농도위10μmol／L。
Objective To explore the inhibition effect of different concentration schisandrin B ( Sch B ) on HepG2 and its mechanism.Methods Cultivating HepG2 cells with 10,25,50,75,100μmol/L Sch B respectively,the MTT method was employed to screened the low toxicity concentration of Sch B .After cultivating with the above gradient con-centration schisandrin B ,the stereochimical structure of HepG 2 cells were observed by invert microscope and its activi-ty was detected by MTT method and western blot was utilized to observe the expression of Hsp -70.The apoptosis con-dition and cell cycle were detected by flow cytometory .Results Sch B had anti-proliferation effect on HepG2 cells growth in dose-dependent manner .The results of flow cytometry showed that the percentage of HepG 2 in sub-G1 phase in 10 μmol/L and 25 μmol/L Sch B group were significantly increased than that in negative control groups ( P<0.05).Conclusion The mechanism of HepG2 cell apoptosis induced by Sch B is probably associated with the down-regulation of Hsp-70 protein expression .The best concentration was 10μmol/L when the Sch B ,as the immuno-modulator ,is combined with other anticancer drug .