CAJ | 학술논문

目的：从细胞凋亡角度探讨黄芪和三七有效成分配伍抗脑缺血再灌注损伤的作用机制。方法将C57 BL／6小鼠随机分为：假手术组、模型组、黄芪甲苷（ ASTⅣ）组、人参皂苷Rg1（ Rg1）组、人参皂苷Rb1（ Rb1）组、三七皂苷R1（ R1）组、四种有效成分配伍组、ASTⅣ＋Rg1组、AST Ⅳ＋Rb1组、AST Ⅳ＋R1组、阳性对照药依达拉奉组，给药3 d后，夹闭双侧颈总动脉造成脑缺血再灌注模型，再灌注24 h后， TUNEL法检测神经细胞凋亡，计算凋亡率，western-blot法测定脑组织caspase-3蛋白表达。结果缺血再灌注后神经细胞凋亡率升高，caspase-3表达上调，各给药组均能降低神经细胞的凋亡率和caspase-3的表达。 ASTⅣ＋Rg1与AST Ⅳ＋R1降低凋亡率和caspase-3表达的效应分别大于各药物单用。四种有效成分配伍组降低凋亡率的效应大于四种有效成分单用及AST Ⅳ＋Rb1，抑制caspase-3蛋白表达的效应大于各有效成分单用及AST Ⅳ＋Rb1、ASTⅣ＋R1。结论黄芪甲苷与三七的主要有效成分配伍能增强对脑缺血再灌注后神经细胞凋亡的抑制作用， ASTⅣ＋Rg1、ASTⅣ＋R1以及四种有效成分配伍对细胞凋亡的抑制作用增强。
목적：종세포조망각도탐토황기화삼칠유효성분배오항뇌결혈재관주손상적작용궤제。방법장C57 BL／6소서수궤분위：가수술조、모형조、황기갑감（ ASTⅣ）조、인삼조감Rg1（ Rg1）조、인삼조감Rb1（ Rb1）조、삼칠조감R1（ R1）조、사충유효성분배오조、ASTⅣ＋Rg1조、AST Ⅳ＋Rb1조、AST Ⅳ＋R1조、양성대조약의체랍봉조，급약3 d후，협폐쌍측경총동맥조성뇌결혈재관주모형，재관주24 h후， TUNEL법검측신경세포조망，계산조망솔，western-blot법측정뇌조직caspase-3단백표체。결과결혈재관주후신경세포조망솔승고，caspase-3표체상조，각급약조균능강저신경세포적조망솔화caspase-3적표체。 ASTⅣ＋Rg1여AST Ⅳ＋R1강저조망솔화caspase-3표체적효응분별대우각약물단용。사충유효성분배오조강저조망솔적효응대우사충유효성분단용급AST Ⅳ＋Rb1，억제caspase-3단백표체적효응대우각유효성분단용급AST Ⅳ＋Rb1、ASTⅣ＋R1。결론황기갑감여삼칠적주요유효성분배오능증강대뇌결혈재관주후신경세포조망적억제작용， ASTⅣ＋Rg1、ASTⅣ＋R1이급사충유효성분배오대세포조망적억제작용증강。
Objective To explore the mechanism of combination of Astragaloside IV and Panax notoginseng active in -gredient against cerebral ischemia-reperfusion injury.Methods C57BL/6 mice were randomly divided into:sham, model,Astragaloside Ⅳ(AST Ⅳ),Ginsenoside Rg1 (Rg1),Ginsenoside Rb1 (Rb1),Notoginsenoside R1 (R1), four active components combination ,ASTⅣ+Rg1,ASTⅣ+Rb1,ASTⅣ+R1 and Edaravone ,pretreated for 3 d. After 1 h of the last administration ,the model of cerebral ischemic-reperfusion injury was established by bilateral com-mon carotid artery (CCA) ligation followed by reperfusion ,then TUNEL method was used to detect apoptosis and ap-optosis rate was calculated ,expressions of caspase-3 proteins in brain tissues were tested by western-blot at 24 h after reperfusion .Results After cerebral ischemia-reperfusion , the apoptosis rate of nerve cell and the expression of caspase-3 protein in brain tissues were increased .All drugs could decrease the apoptosis rate ,inhibit caspase-3 pro-tein expression .Furthermore , the decreased effects of AST Ⅳ+Rg1 and AST Ⅳ+R1 on the apoptosis rate and caspase-3 protein expression were better than those of the active components alone;In four active components combina-tion,the decrease of the apoptosis rate was stronger than that of four active components alone and AST Ⅳ+Rb1,the inhibition of caspase-3 was greater than that of four active components alone and AST Ⅳ+Rb1,ASTⅣ+R1.Con-clusion AST Ⅳcombined with the effective components of Panax notoginseng had the potentiation on the inhibition of apoptosis .The inhibitory effect of AST Ⅳ+Rg1 ,AST Ⅳ+R1 and four kinds of active ingredient combination on cell apoptosis are enhanced .