CAJ | 학술논문

为了提高美藤果油的提取率以及油的品质，该文以美藤果为原料，比较了水酶法、超临界CO2萃取法、超声波辅助有机溶剂萃取法和低温冷榨法这4种不同的提取方法对美藤果油提取率和油品质的影响；并采用气相色谱-质谱联用法（gas chromatograph-mass spectrometer-computer, GC-MS）和高效液相色谱法（high performance liquid chromatography, HPLC）测定美藤果油的营养组成，以及以1,1-二苯基-2-三硝基苯肼（1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl，DPPH?自由基）清除率为指标比较了美藤果油与其他植物油的体外抗氧化活性。结果表明：4种提取方法中，水酶法和超声波辅助有机溶剂提取法油脂提取率较高，分别为95.1%和92.7%，其次为超临界萃取法，为86.44%。但超临界CO2萃取法提取出的油脂综合理化指标优于其他3种提取方法，其中水酶法提取出的油不溶性杂质、酸价、过氧化值和黄色值都低于超声波辅助有机溶剂萃取法和低温冷榨法，但感官品质最差。综合考虑得出：超临界萃取法为美藤果油较佳的提取方法。此方法提取的美藤果油中富含不饱和脂肪酸和生育酚、多酚等活性成分，不饱和脂肪酸质量分数高达92.37%，总生育酚和总多酚质量分数分别为59.2 mg/(100 g)、10.2 mg/(100 g)，但不含有维生素A；美藤果油对DPPH·自由基的半数抑制率IC50为1.148 mg/mL，明显低于橄榄油、茶油、亚麻籽油和紫苏油（P<0.01）,说明美藤果油体外抗氧化能力较强。研究结果可为美藤果油进一步开发提供参考。
위료제고미등과유적제취솔이급유적품질，해문이미등과위원료，비교료수매법、초림계CO2췌취법、초성파보조유궤용제췌취법화저온랭자법저4충불동적제취방법대미등과유제취솔화유품질적영향；병채용기상색보-질보련용법（gas chromatograph-mass spectrometer-computer, GC-MS）화고효액상색보법（high performance liquid chromatography, HPLC）측정미등과유적영양조성，이급이1,1-이분기-2-삼초기분정（1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl，DPPH?자유기）청제솔위지표비교료미등과유여기타식물유적체외항양화활성。결과표명：4충제취방법중，수매법화초성파보조유궤용제제취법유지제취솔교고，분별위95.1%화92.7%，기차위초림계췌취법，위86.44%。단초림계CO2췌취법제취출적유지종합이화지표우우기타3충제취방법，기중수매법제취출적유불용성잡질、산개、과양화치화황색치도저우초성파보조유궤용제췌취법화저온랭자법，단감관품질최차。종합고필득출：초림계췌취법위미등과유교가적제취방법。차방법제취적미등과유중부함불포화지방산화생육분、다분등활성성분，불포화지방산질량분수고체92.37%，총생육분화총다분질량분수분별위59.2 mg/(100 g)、10.2 mg/(100 g)，단불함유유생소A；미등과유대DPPH·자유기적반수억제솔IC50위1.148 mg/mL，명현저우감람유、다유、아마자유화자소유（P<0.01）,설명미등과유체외항양화능력교강。연구결과가위미등과유진일보개발제공삼고。
Sacha inchi is an ancient plant existing in highland in Ecuador and Peru. It is a good oil source containing many unsaturated fatty acids, which are beneficial to our health. In present, the study of Sacha inchi has been focused on its nutritional composition, oil stability and cultivation techniques. The study on different kinds of extracting oil methods of Sacha inchi has not been reported. Moreover, the reports of Sacha inchi were usually from Ecuador and Peru and other countries abroad, but that from China was seldom seen. So the purpose of this paper was to study the extraction oil methods and the nutritional composition of Sacha inchi which was provided by Puer Lianzhong Biological Resources Development Limited Company in Yunnan Province, China. Sacha inchis were used as raw material in order to compare the oil extraction efficiency and oil quality among 4 kinds of extraction methods, which were aqueous enzymatic extraction, supercritical CO2 extraction, ultrasonic-assisted organic solvent extraction and low-temperature cold pressing method. In this processing, oil extraction efficiency and oil quality including color, transparency, smell and taste as well as the physical and chemical indices of Sacha inchi oil were determined. Furthermore, fatty acid and other nutritional compositions of the oil were determined by GC-MS (gas chromatograph-mass spectrometer-computer) and HPLC (high performance liquid chromatography), respectively. The elimination rate of DPPH (1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl) free radical was determined to evaluate the antioxidant activity in vitro between Sacha inchi oil and other vegetable oils. The results showed that although the oil extraction efficiency of supercritical CO2 extraction was lower than that of aqueous enzymatic extraction and ultrasonic-assisted organic solvent extraction, it was still the best extraction method among these 4 methods due to its good oil quality. The color, insoluble impurities, moisture and volatiles by hot plate, acid value and peroxide value of the oil extracted by supercritical CO2 method were significantly lower (P<0.05) than ultrasonic-assisted organic solvent extraction, and the color, insoluble impurities, acid value and peroxide value were also significantly lower (P<0.05) than low-temperature cold pressing method. The oil obtained by aqueous enzymatic extraction had the lowest acid value and peroxide value compared to the other 3 methods, but the smell and taste of oil were relatively light. The Sacha inchi oil is rich in unsaturated fatty acids and tocopherol, polyphenols and other active ingredients. The unsaturated fatty acids containα-linolenic acid, linoleic acid, oleic acid and so on, and their contents were up to 92.37% of total lipids. The content ofα-linolenic acid was 43.7% of total lipids which was higher than that of blackberry seed oil (8.38% of total lipids) or rapeseed oil (9% of total lipids). The content of linoleic acid was also high, which made Sacha inchi oil have a good proportion between omega-6 and omega-3 (about 1:1). The content of total tocopherol and total polyphenols were 59.2 and 10.2 mg/(100g), respectively, and among them (β+γ)-tocopherol was high to 41.2 mg/(100g), which made Sacha inchi oil more stable than flaxseed oil whose unsaturated fatty acids were also high. However, vitamin A was not detected in Sacha inchi oil. The 50% inhibitory rate (the required concentration of antioxidant substances when the removal rate was 50%) for Sacha inchi oil was 1.148 mg/mL, which was only 13%, 1%, 11% and 13% of olive oil, tea oil, flaxseed oil and perilla oil, respectively. This indicated that the antioxidant capacity in vitro for Sacha inchi oil was obviously better than that of olive oil, tea oil, flaxseed oil and perilla oil. In conclusion, supercritical CO2 extraction is the best way to improve oil quality of Sacha inchi and Sacha inchi oil is a good source of polyunsaturated fatty acids.