河北医药
河北醫藥
하북의약
Hebei Medical Journal
2015年
21期
3205-3208
,共4页
胰腺癌%环氧合酶-2%血管内皮生长因子%基质金属蛋白酶-9%塞来布昔
胰腺癌%環氧閤酶-2%血管內皮生長因子%基質金屬蛋白酶-9%塞來佈昔
이선암%배양합매-2%혈관내피생장인자%기질금속단백매-9%새래포석
pancreatic cancer%cyclooxygease 2%vascular endothelial growth factor%matrix metalloproteinase 9%celecoxib
目的：观察胰腺癌组织环氧合酶-2（COX-2）、血管内皮生长因子（VEGF）、基质金属蛋白酶-9（MMP-9）的表达及塞来布昔对人胰腺癌BXPC-3细胞VEGF、MMP-9表达的影响。方法采用免疫组织化学SP法检测COX-2、VEGF、MMP-9在50例原发性胰腺癌及癌旁正常组织的表达，分析其与胰腺癌临床病理特征的关系。实时荧光定量PCR法（ RT-QPCR）和Western blot检测塞来布昔对BXPC-3细胞VEGF、MMP-9 mRNA和蛋白表达的影响。结果COX-2、VEGF、MMP-9在胰腺癌组织的阳性表达率显著高于癌旁组织，差异有统计学意义（ P ＜0 V．05）。 COX-2、VEGF、 MMP-9高表达与胰腺癌增殖活性、TNM临床分期及肿瘤浸润转移等临床病理特征相关（ P ＜0．05），而与年龄、性别、发病部位、病理组织分级无关（ P ＞0．05）；且COX-1与VEGF、MMP-9表达呈正相关性（ P ＜0．05）。随着塞来布昔剂量的增高，BXPC-3细胞VEGF、MMP-9 mRNA和蛋白表达均降低，与对照组比较差异有统计学意义（ P ＜0．05）。结论 COX-2、VEGF、MMP-9胰腺癌组织表达增高，可能在肿瘤发生发展中发挥重要作用；COX-2特异性抑制剂塞来布昔可能通过下调VEGF、MMP-9的表达发挥抗肿瘤作用。
目的：觀察胰腺癌組織環氧閤酶-2（COX-2）、血管內皮生長因子（VEGF）、基質金屬蛋白酶-9（MMP-9）的錶達及塞來佈昔對人胰腺癌BXPC-3細胞VEGF、MMP-9錶達的影響。方法採用免疫組織化學SP法檢測COX-2、VEGF、MMP-9在50例原髮性胰腺癌及癌徬正常組織的錶達，分析其與胰腺癌臨床病理特徵的關繫。實時熒光定量PCR法（ RT-QPCR）和Western blot檢測塞來佈昔對BXPC-3細胞VEGF、MMP-9 mRNA和蛋白錶達的影響。結果COX-2、VEGF、MMP-9在胰腺癌組織的暘性錶達率顯著高于癌徬組織，差異有統計學意義（ P ＜0 V．05）。 COX-2、VEGF、 MMP-9高錶達與胰腺癌增殖活性、TNM臨床分期及腫瘤浸潤轉移等臨床病理特徵相關（ P ＜0．05），而與年齡、性彆、髮病部位、病理組織分級無關（ P ＞0．05）；且COX-1與VEGF、MMP-9錶達呈正相關性（ P ＜0．05）。隨著塞來佈昔劑量的增高，BXPC-3細胞VEGF、MMP-9 mRNA和蛋白錶達均降低，與對照組比較差異有統計學意義（ P ＜0．05）。結論 COX-2、VEGF、MMP-9胰腺癌組織錶達增高，可能在腫瘤髮生髮展中髮揮重要作用；COX-2特異性抑製劑塞來佈昔可能通過下調VEGF、MMP-9的錶達髮揮抗腫瘤作用。
목적：관찰이선암조직배양합매-2（COX-2）、혈관내피생장인자（VEGF）、기질금속단백매-9（MMP-9）적표체급새래포석대인이선암BXPC-3세포VEGF、MMP-9표체적영향。방법채용면역조직화학SP법검측COX-2、VEGF、MMP-9재50례원발성이선암급암방정상조직적표체，분석기여이선암림상병리특정적관계。실시형광정량PCR법（ RT-QPCR）화Western blot검측새래포석대BXPC-3세포VEGF、MMP-9 mRNA화단백표체적영향。결과COX-2、VEGF、MMP-9재이선암조직적양성표체솔현저고우암방조직，차이유통계학의의（ P ＜0 V．05）。 COX-2、VEGF、 MMP-9고표체여이선암증식활성、TNM림상분기급종류침윤전이등림상병리특정상관（ P ＜0．05），이여년령、성별、발병부위、병리조직분급무관（ P ＞0．05）；차COX-1여VEGF、MMP-9표체정정상관성（ P ＜0．05）。수착새래포석제량적증고，BXPC-3세포VEGF、MMP-9 mRNA화단백표체균강저，여대조조비교차이유통계학의의（ P ＜0．05）。결론 COX-2、VEGF、MMP-9이선암조직표체증고，가능재종류발생발전중발휘중요작용；COX-2특이성억제제새래포석가능통과하조VEGF、MMP-9적표체발휘항종류작용。
Objective To investigate the expressions of cyclooxygenase-2 ( COX-2 ) , vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9) in pancreatic cancer tissue and the effects of celecoxib on expression of VEGF and MMP-9 in human pancreatic cancer cell line BXPC-3.Methods The expression levels of COX-2, VEGF, MMP-9 proteins were detected by immunohistochemistry ( SP) in 50 cases of primary pancreatic cancer tissue and adjacent normal tissues,moreover, the correlation between the expressions of COX-2, VEGF, MMP-9 and clinicopathological characteristics of pancreatic cancer was analyzed.The effects of celecoxib on expressions of VEGF and MMP-9 mRNA and proteins in human pancreatic cancer cell line BXPC-3 were detected by RT-QPCR and Western Blot.Results The expression levels of COX-2, VEGF, MMP-9 in pancreatic cancer tissue were significantly higher than those in adjacent normal tissue ( P <0.05 ) .The expressions of COX-2, VEGF, MMP-9 were closely correlated to proliferative activity, tumor metastasis and clinical stage of pancreatic carcinoma ( P <0.05), however,which were not related with patient’ s age and gender, anatomical site and histological grade of pancreatic carcinoma,furthermore, the expression of COX-2 was positively correlated to that of VEGF or MMP-9 ( P <0.05).With the increase of celecoxib dosage ,the expression levels of VEGF, MMP-9 mRNA and protein in pancreatic cancer cell line BXPC-3were significantly decreased , as compared with those in control group ( P <0.05 ) . Conclusion The high expression of COX-2, VEGF, MMP-9 in pancreatic carcinoma may play an important role in pathogenesis and progression of pancreatic carcinoma.Celecoxib may develop antitumor effects through down-regulating the expressions of COX-2, MMP-9 and VEGF.
