CAJ | 학술논문

为降低鸡场肠病原体疫病传播风险和兽药残留,通过响应面中和设计法,该试验评估了微酸性电解水(pH值 5.85~6.53)对大肠杆菌和沙门氏菌混合污染轮胎的消毒效果,并探讨了清洗时间,消毒时间及有效氯浓度3个因素对微酸性电解水消毒效果的影响及相互作用规律,同时建立二次多项回归模型,并对消毒工艺进行优化.结果表明,3 个因素皆对消毒效果有显著影响(p<0.0001),且各因素影响大小为有效氯浓度>消毒时间>清洗时间;模型决定系数和调整决定系数分别为0.984和0.969,验证试验中,试验值与预测值的相关系数为0.97;消毒时间5 min、清洗时间4 min、有效氯浓度140 mg/L时,可以达到1.38 log10cfu/cm2的杀菌数.该研究为微酸性电解水消毒提供了参考,并证明了微酸性电解水在畜牧业的应用潜力.
위강저계장장병원체역병전파풍험화수약잔류,통과향응면중화설계법,해시험평고료미산성전해수(pH치 5.85~6.53)대대장간균화사문씨균혼합오염륜태적소독효과,병탐토료청세시간,소독시간급유효록농도3개인소대미산성전해수소독효과적영향급상호작용규률,동시건립이차다항회귀모형,병대소독공예진행우화.결과표명,3 개인소개대소독효과유현저영향(p<0.0001),차각인소영향대소위유효록농도>소독시간>청세시간;모형결정계수화조정결정계수분별위0.984화0.969,험증시험중,시험치여예측치적상관계수위0.97;소독시간5 min、청세시간4 min、유효록농도140 mg/L시,가이체도1.38 log10cfu/cm2적살균수.해연구위미산성전해수소독제공료삼고,병증명료미산성전해수재축목업적응용잠력.
The process of transport has long been considered an important risk factor for pathogens entry into farms. Disinfection is a generally agreed concept to prevent the introduction of both endemic and epidemic infections, however, potentially toxic, corrosive or volatile problems have arisen because of the use of chemicals as disinfecting agents. Slightly acidic electrolyzed water (SAEW) is considered environmental friendly as it is generated from water and dilute salt solution and reverts to water after use. Also, it has the advantages of possessing broad-spectrum antimicrobial activity, reducing corrosion of surface and minimizing the potential of damage to human health. To reduce the risk of enteric pathogens transmission and leave little residue in animal farms, the disinfection effectiveness of SAEW with pH value of 5.85-6.53 for inactivatingEscherichia coli (E. coli) andSalmonella enteritidis (S. enteritidis) mixture on the surface of vehicle tires was evaluated. The coupled effects of tap water washing time (from 2 to 4 min), SAEW treatment time (from 3 to 7 min) and available chlorine concentration (ACC) (from 80 to 140 mg/L) on the reduction ofE. coli and S. enteritidis mixture on tires were investigated using a central composite design of the response surface (RS) methodology. The established RS model had a good fitting quantified by determination coefficient (R2) of 0.984 and adjusted determination coefficient of 0.969 (p>0.05). The model was validated with additional random 8 conditions within the experimental domain. The predicted value showed a good agreement with the actual values, for the points of response values were very close to the line of 100% correlation. The results showed that the cleaning time, disinfection time and ACC significantly affected the pathogens reduction (p<0.0001), and their influences were ranked as ACC>disinfection time>cleaning time. The linear correlation coefficients, the quadratic term coefficients and the cross validation coefficients between cleaning time and disinfection time, cleaning time and ACC were significant (p<0.05). The other term coefficient between ACC and disinfection time was not significant. The more reduction of pathogens and the significant interactions between cleaning time and other factors were likely due to the livestock manure, which was a strong limiting factor for disinfection of SAEW. Several authors have stated that the organic soiling could change the formation of combined available chlorines to affect the disinfection effectiveness of SAEW. Therefore, if livestock manure could be more removed by more cleaning time along with more SAEW treatment time and higher ACC, a more effective disinfection would be obtained. Therefore, cleaning time is very important for SAEW disinfection when organic matters exist. The maximum reduction of 1.38 log10cfu/cm2 (92.9%) forE. coli andS. enteritidis mixture was obtained for the vehicle tire washed with tap water for 4 min followed by SAEW treatment for 5 min at an ACC of 140 mg/L. The established RS model could be used. The result proves the potential of the SAEW in disinfection of bacterial cells on tires and in promoting the implementation of disinfection measures to control and reduce the transmission risk of the disease.