实用药物与临床
實用藥物與臨床
실용약물여림상
Practical Pharmacy and Clinical Remedies
2015年
10期
1143-1146
,共4页
韦晓勇%王毅%闵红星%陆波%李军
韋曉勇%王毅%閔紅星%陸波%李軍
위효용%왕의%민홍성%륙파%리군
七氟醚%认知障碍%海马%突触%异氟醚
七氟醚%認知障礙%海馬%突觸%異氟醚
칠불미%인지장애%해마%돌촉%이불미
Sevoflurane%Cognition disorders%Hippocampus%Synapses%Isoflurane
目的 观察七氟醚对老龄大鼠海马CA3区突触结构的影响.方法 健康SD大鼠60 只,18 月龄,随机分为4组:空白对照组(C组)、3%七氟醚组(S1 组)、1.5%七氟醚组(S2 组)、3%异氟醚组(Ⅰ组),每组15只.于麻醉结束后1、3、7 d ( T1 ~T3 )行Morris水迷宫实验,并测定海马CA3区突触结构各指标.结果 与C组比较,Ⅰ组T1 ~T3、S1 组T1 ~T2 时逃避潜伏期延长,穿越原平台象限次数减少,Ⅰ组、S1 组T1 ~T3 海马CA3区突触间隙增宽,PSD厚度变薄,突触活性区长度缩短,突触界面曲率减小( P <0.05 );与 Ⅰ 组、S1 组比较,S2 组T1 ~T2时逃避潜伏期缩短,T1 ~T3 海马CA3区突触间隙变窄,PSD厚度增厚,突触活性区长度延长,突触界面曲率增大(P<0.05);与I组比较,S1 组、S2 组T3 时逃避潜伏期缩短,穿越原平台象限次数增加(P<0.05);与S1 组比较,I组T3 时逃避潜伏期延长,穿越原平台象限次数减少(P<0.05);与T1 比较,Ⅰ组、S1 组、S2 组T2 ~T3 时逃避潜伏期缩短(P<0.05).结论 低浓度七氟醚对老龄大鼠认知功能无明显影响,高浓度七氟醚、异氟醚均可导致老龄大鼠认知功能减退,其机制可能与抑制海马CA3区突触结构的改变有关.
目的 觀察七氟醚對老齡大鼠海馬CA3區突觸結構的影響.方法 健康SD大鼠60 隻,18 月齡,隨機分為4組:空白對照組(C組)、3%七氟醚組(S1 組)、1.5%七氟醚組(S2 組)、3%異氟醚組(Ⅰ組),每組15隻.于痳醉結束後1、3、7 d ( T1 ~T3 )行Morris水迷宮實驗,併測定海馬CA3區突觸結構各指標.結果 與C組比較,Ⅰ組T1 ~T3、S1 組T1 ~T2 時逃避潛伏期延長,穿越原平檯象限次數減少,Ⅰ組、S1 組T1 ~T3 海馬CA3區突觸間隙增寬,PSD厚度變薄,突觸活性區長度縮短,突觸界麵麯率減小( P <0.05 );與 Ⅰ 組、S1 組比較,S2 組T1 ~T2時逃避潛伏期縮短,T1 ~T3 海馬CA3區突觸間隙變窄,PSD厚度增厚,突觸活性區長度延長,突觸界麵麯率增大(P<0.05);與I組比較,S1 組、S2 組T3 時逃避潛伏期縮短,穿越原平檯象限次數增加(P<0.05);與S1 組比較,I組T3 時逃避潛伏期延長,穿越原平檯象限次數減少(P<0.05);與T1 比較,Ⅰ組、S1 組、S2 組T2 ~T3 時逃避潛伏期縮短(P<0.05).結論 低濃度七氟醚對老齡大鼠認知功能無明顯影響,高濃度七氟醚、異氟醚均可導緻老齡大鼠認知功能減退,其機製可能與抑製海馬CA3區突觸結構的改變有關.
목적 관찰칠불미대노령대서해마CA3구돌촉결구적영향.방법 건강SD대서60 지,18 월령,수궤분위4조:공백대조조(C조)、3%칠불미조(S1 조)、1.5%칠불미조(S2 조)、3%이불미조(Ⅰ조),매조15지.우마취결속후1、3、7 d ( T1 ~T3 )행Morris수미궁실험,병측정해마CA3구돌촉결구각지표.결과 여C조비교,Ⅰ조T1 ~T3、S1 조T1 ~T2 시도피잠복기연장,천월원평태상한차수감소,Ⅰ조、S1 조T1 ~T3 해마CA3구돌촉간극증관,PSD후도변박,돌촉활성구장도축단,돌촉계면곡솔감소( P <0.05 );여 Ⅰ 조、S1 조비교,S2 조T1 ~T2시도피잠복기축단,T1 ~T3 해마CA3구돌촉간극변착,PSD후도증후,돌촉활성구장도연장,돌촉계면곡솔증대(P<0.05);여I조비교,S1 조、S2 조T3 시도피잠복기축단,천월원평태상한차수증가(P<0.05);여S1 조비교,I조T3 시도피잠복기연장,천월원평태상한차수감소(P<0.05);여T1 비교,Ⅰ조、S1 조、S2 조T2 ~T3 시도피잠복기축단(P<0.05).결론 저농도칠불미대노령대서인지공능무명현영향,고농도칠불미、이불미균가도치노령대서인지공능감퇴,기궤제가능여억제해마CA3구돌촉결구적개변유관.
Objective To observe the effects of sevoflurane on the synaptic interface structure in hippocampal CA3 area in senile rats.Methods Sixty healthy SD rats aged 18 months were randomly divided into 4 groups:blank control group(group C,n=15),3% sevoflurance group (group S1,n=15),1.5% sevoflurance group (group S2,n=15),3% isoflurane group (group Ⅰ,n=15).The Morris water maze test was performed at the 1st,3rd and 7th day ( T1 ~T3 ) after anesthesia,and the synaptic structure in hippocampal CA3 area was measured.Results Compared with group C,the escape latency was longer,the frequency of crossing the original platform was reduced at T1 ~T3 in group Ⅰ and at T1 ~T2 in group S1,the width of synaptic cleft increased,the thickness of the postsynaptic density de-creased,the length of active zones was shortened,and the curvature of the synaptic interface decreased at T1 ~T3 in group Ⅰ and group S1(P<0.05);Compared with group Ⅰ and group S1,the escape latency was shortened at T1 ~T2 in group S2,the width of synaptic cleft decreased,the thickness of the postsynaptic density increased,the length of active zones was prolonged,and the curvature of the synaptic interface was increased at T1 ~T3 in group S2(P<0.05);Com-pared with group Ⅰ,the escape latency was shortened,the frequency of crossing the original platform increased at T3 in group S1 and group S2(P<0.05);Compared with group S1,the escape latency was longer,the frequency of crossing the original platform was reduced at T3 in group Ⅰ (P<0.05);Compared with T1,the escape latency was shortened at T2 ~T3 in group Ⅰ,group S1 and group S2(P<0.05).Conclusion Sevoflurane of low concentration has no obvious effect on cognitive function in senile rats.Both sevoflurane and isoflurane of high concentration can induce the cogni-tive hypofunction in senile rats,and the mechanism may be associated with the inhibition of the changes of synaptic structure in hippocampal CA3 area.
