CAJ | 학술논문

目的：建立一种敏感性高、特异性强的检测胆螺杆菌的套式PCR方法。方法基于17种胆螺杆菌亚种的16S rRNA 基因设计筛选出1套套式引物（2条内引物、2条外引物），优化反应条件后，通过模拟粪便标本、动物模型标本和临床标本的检测对方法的敏感性和特异性进行评估。结果模拟粪便标本中，该方法检测胆螺杆菌的敏感性达到10 CFU／100μL。3例感染胆螺杆菌的SPF级BALB／c小鼠模型中，3例小鼠粪便和盲肠中均可检测到胆螺杆菌，1例肝脏标本中检测到了胆螺杆菌。10例胆石病患者中，有2例患者的胆汁、胆囊粘膜和粪便标本中检测到了胆螺杆菌。结论本研究建立的套式 PCR方法，敏感性高、特异性强，可用于检测胆螺杆菌的感染。
목적：건립일충민감성고、특이성강적검측담라간균적투식PCR방법。방법기우17충담라간균아충적16S rRNA 기인설계사선출1투투식인물（2조내인물、2조외인물），우화반응조건후，통과모의분편표본、동물모형표본화림상표본적검측대방법적민감성화특이성진행평고。결과모의분편표본중，해방법검측담라간균적민감성체도10 CFU／100μL。3례감염담라간균적SPF급BALB／c소서모형중，3례소서분편화맹장중균가검측도담라간균，1례간장표본중검측도료담라간균。10례담석병환자중，유2례환자적담즙、담낭점막화분편표본중검측도료담라간균。결론본연구건립적투식 PCR방법，민감성고、특이성강，가용우검측담라간균적감염。
In this study ,the objective is to establish a nested‐PCR assay for the detection of H .bilis with high sensitivity and specificity .The nested primers were designed based on sequences of 16S rRNA gene of seventeen subtypes of H .bilis .Af‐ter optimizing reaction condition ,the sensitivity and specificity of the assay were examined via the detection of feces simulated samples ,mice infection model samples and clinic patients’ samples .The detection sensitivity of H .bilis strain for feces simu‐lated samples was 10 CFU/100 μL .H .bilis was successfully detected in the liver ,caecum and feces of experimentally infected mice .Moreover ,H .bilis was successfully detected in the bile ,cholecyst mucous membrane and feces samples from two of ten patients with cholelithiasis .Due to the PCR assay’s high sensitivity and specificity ,the method may be used to detect the infec‐tion of H .bilis .