国际药学研究杂志
國際藥學研究雜誌
국제약학연구잡지
Journal of International Pharmaceutical Research
2015年
5期
637-641
,共5页
欧亮苗%阿来·赛坎%李柯翱%季志红%田树革
歐亮苗%阿來·賽坎%李柯翱%季誌紅%田樹革
구량묘%아래·새감%리가고%계지홍%전수혁
没食子%没食子酸%没食子酸甲酯%鞣花酸%高效液相色谱法
沒食子%沒食子痠%沒食子痠甲酯%鞣花痠%高效液相色譜法
몰식자%몰식자산%몰식자산갑지%유화산%고효액상색보법
Quercus infectoria Olivier%gallic acid%methyl gallate%ellagic acid%HPLC
目的 建立维药没食子中没食子酸、没食子酸甲酯和鞣花酸的HPLC含量测定的方法,并检测10批没食子中没食子酸、没食子酸甲酯和鞣花酸的含量. 方法 采用高效液相色谱法,Agilent Zorbax SB-C18柱(4.6 mm × 250 mm,5μm),流动相甲醇(A)-0.05%磷酸水溶液(B)进行梯度洗脱,流速1 ml/min,进样量5 μl, 检测波长258 nm.结果 没食子酸的质量浓度在0.0318 ~ 0.1871 g/L(r=0.9994)呈良好的线性关系,平均回收率为97.52%,RSD为1.41%;没食子酸甲酯的质量浓度在0 . 0769~ 0 . 4612 g/L(r=0 . 9994)呈良好的线性关系,平均回收率为99 . 15%, RSD为1 . 46%;鞣花酸的质量浓度在0 . 0158~0.0553 g/L(r=0.9998)呈良好的线性关系,平均回收率为102.75%,RSD为0.87%.结论 本方法简便、快速、准确、重复性好,为维药没食子质量控制提供定量依据.
目的 建立維藥沒食子中沒食子痠、沒食子痠甲酯和鞣花痠的HPLC含量測定的方法,併檢測10批沒食子中沒食子痠、沒食子痠甲酯和鞣花痠的含量. 方法 採用高效液相色譜法,Agilent Zorbax SB-C18柱(4.6 mm × 250 mm,5μm),流動相甲醇(A)-0.05%燐痠水溶液(B)進行梯度洗脫,流速1 ml/min,進樣量5 μl, 檢測波長258 nm.結果 沒食子痠的質量濃度在0.0318 ~ 0.1871 g/L(r=0.9994)呈良好的線性關繫,平均迴收率為97.52%,RSD為1.41%;沒食子痠甲酯的質量濃度在0 . 0769~ 0 . 4612 g/L(r=0 . 9994)呈良好的線性關繫,平均迴收率為99 . 15%, RSD為1 . 46%;鞣花痠的質量濃度在0 . 0158~0.0553 g/L(r=0.9998)呈良好的線性關繫,平均迴收率為102.75%,RSD為0.87%.結論 本方法簡便、快速、準確、重複性好,為維藥沒食子質量控製提供定量依據.
목적 건립유약몰식자중몰식자산、몰식자산갑지화유화산적HPLC함량측정적방법,병검측10비몰식자중몰식자산、몰식자산갑지화유화산적함량. 방법 채용고효액상색보법,Agilent Zorbax SB-C18주(4.6 mm × 250 mm,5μm),류동상갑순(A)-0.05%린산수용액(B)진행제도세탈,류속1 ml/min,진양량5 μl, 검측파장258 nm.결과 몰식자산적질량농도재0.0318 ~ 0.1871 g/L(r=0.9994)정량호적선성관계,평균회수솔위97.52%,RSD위1.41%;몰식자산갑지적질량농도재0 . 0769~ 0 . 4612 g/L(r=0 . 9994)정량호적선성관계,평균회수솔위99 . 15%, RSD위1 . 46%;유화산적질량농도재0 . 0158~0.0553 g/L(r=0.9998)정량호적선성관계,평균회수솔위102.75%,RSD위0.87%.결론 본방법간편、쾌속、준학、중복성호,위유약몰식자질량공제제공정량의거.
Objective To dertermine the content of gallic acid, methyl gallate and ellagic acid in galls of Quercus infectoria Olivier from different batches by HPLC. Methods The chromatogram colume was Agilent Zorbax SB-C18(4.6 mm × 250 mm, 5μm), gradiently eluting with methanol as mobile phase A and 0.01%phosphoric acid as B at a flow rate of 1 ml/min. The injection volumn was 5 μl. Detection wavelength was at 258 nm. Results Gallic acid was linear in the range of 0.0318-0.1871 g/L with correlation coefficient of 0.9994. The average recovery was 97.52%with RSD 1.41%. Methyl gallate was linear in the range of 0.0769-0.4612 g/L with correlation coefficient of 0.9994. The average recovery was 99.15% with RSD at 1.46%. Ellagic acid was linear in the range of 0.0158-0.0553 g/L with correlation coefficient of 0.9998. The average recovery was 102.75% with RSD at 0.87%. Conclusion The method is convenient, fast, accurate and practicable, and can be used for controling the quality of the galls of Q. infectoria.
