临床肿瘤学杂志
臨床腫瘤學雜誌
림상종류학잡지
Chinese Clinical Oncology
2015年
10期
875-878
,共4页
S期激酶相关蛋白%p27%p21%肺癌%移植瘤
S期激酶相關蛋白%p27%p21%肺癌%移植瘤
S기격매상관단백%p27%p21%폐암%이식류
S-phase kinase associated protein%p27%p21%Lung cancer%Neoplasm transplantation
目的：探讨抑制Skp2基因表达对裸鼠肺癌移植瘤生长以及移植瘤组织中Skp2、p27、p21蛋白表达的影响。方法构建抑制Skp2基因表达的重组质粒Skp2 shRNA 1和Skp2 shRNA 2，以及Skp2 shRNA HK重组质粒（阴性pGensil?HK对照质粒）。3种重组质粒分别转染肺癌SPC?A?1细胞，提取该细胞悬液于裸鼠皮下注射建立肺癌转染细胞裸鼠动物模型。观察肿瘤形成时间，测量其体积，绘制肿瘤生长曲线。裸鼠接种转染肺癌细胞30天后处死，提取移植瘤组织并计算抑瘤率， Western blotting检测移植瘤组织中Skp2、p27、p21蛋白的表达。结果转染Skp2 shRNA 1和Skp2 shRNA 2质粒的裸鼠肿瘤生长明显减慢。与对照组和Skp2 shRNA HK组比较，Skp2 shRNA 1和Skp2 shRNA 2质粒组肿瘤体积明显缩小（ P＜0?05），抑瘤率分别为（69?2±5?6）％和（64?5±6?1）％。与对照组比较， Skp2 shRNA 1和Skp2 shRNA 2质粒组Skp2蛋白下调率为（73?5±4?2）％、（67?3±3?2）％， p27蛋白上调率为（63?6±2?2）％、（57?3±2?4）％，p21蛋白上调率为（54?6±3?4）％、（51?7±3?1）％。结论抑制Skp2基因表达可明显抑制移植瘤生长，Skp2基因有望成为肺癌基因治疗的新靶点。
目的：探討抑製Skp2基因錶達對裸鼠肺癌移植瘤生長以及移植瘤組織中Skp2、p27、p21蛋白錶達的影響。方法構建抑製Skp2基因錶達的重組質粒Skp2 shRNA 1和Skp2 shRNA 2，以及Skp2 shRNA HK重組質粒（陰性pGensil?HK對照質粒）。3種重組質粒分彆轉染肺癌SPC?A?1細胞，提取該細胞懸液于裸鼠皮下註射建立肺癌轉染細胞裸鼠動物模型。觀察腫瘤形成時間，測量其體積，繪製腫瘤生長麯線。裸鼠接種轉染肺癌細胞30天後處死，提取移植瘤組織併計算抑瘤率， Western blotting檢測移植瘤組織中Skp2、p27、p21蛋白的錶達。結果轉染Skp2 shRNA 1和Skp2 shRNA 2質粒的裸鼠腫瘤生長明顯減慢。與對照組和Skp2 shRNA HK組比較，Skp2 shRNA 1和Skp2 shRNA 2質粒組腫瘤體積明顯縮小（ P＜0?05），抑瘤率分彆為（69?2±5?6）％和（64?5±6?1）％。與對照組比較， Skp2 shRNA 1和Skp2 shRNA 2質粒組Skp2蛋白下調率為（73?5±4?2）％、（67?3±3?2）％， p27蛋白上調率為（63?6±2?2）％、（57?3±2?4）％，p21蛋白上調率為（54?6±3?4）％、（51?7±3?1）％。結論抑製Skp2基因錶達可明顯抑製移植瘤生長，Skp2基因有望成為肺癌基因治療的新靶點。
목적：탐토억제Skp2기인표체대라서폐암이식류생장이급이식류조직중Skp2、p27、p21단백표체적영향。방법구건억제Skp2기인표체적중조질립Skp2 shRNA 1화Skp2 shRNA 2，이급Skp2 shRNA HK중조질립（음성pGensil?HK대조질립）。3충중조질립분별전염폐암SPC?A?1세포，제취해세포현액우라서피하주사건립폐암전염세포라서동물모형。관찰종류형성시간，측량기체적，회제종류생장곡선。라서접충전염폐암세포30천후처사，제취이식류조직병계산억류솔， Western blotting검측이식류조직중Skp2、p27、p21단백적표체。결과전염Skp2 shRNA 1화Skp2 shRNA 2질립적라서종류생장명현감만。여대조조화Skp2 shRNA HK조비교，Skp2 shRNA 1화Skp2 shRNA 2질립조종류체적명현축소（ P＜0?05），억류솔분별위（69?2±5?6）％화（64?5±6?1）％。여대조조비교， Skp2 shRNA 1화Skp2 shRNA 2질립조Skp2단백하조솔위（73?5±4?2）％、（67?3±3?2）％， p27단백상조솔위（63?6±2?2）％、（57?3±2?4）％，p21단백상조솔위（54?6±3?4）％、（51?7±3?1）％。결론억제Skp2기인표체가명현억제이식류생장，Skp2기인유망성위폐암기인치료적신파점。
Objective To investigate the influence of suppressing Skp2 gene expression on growth of lung cancer xenografts and Skp2, p27, p21 protein expression in xenografts. Methods BALB/c nude mice were injected subcutaneously with SPC?A?1 cells transfected with Skp2 shRNA 1,Skp2 shRNA 2 and Skp2 shRNA HK to construct nude mice of lung cancer animal models. Time of tumor formation and volume of tumors were observed. The mice were sacrificed and xenografts tissues were taken, and tumor inhibition rate was caculated. Protein expression of Skp2,p27, p21 in xenografts tissues was detected by Western blotting method. Results Com?pared with the control group and Skp2 shRNA HK group, tumor growth were inhibited and tumor volume reduced significantly in Skp2 shRNA 1 and Skp2 shRNA 2 groups. Tumor inhibition rate of Skp2 shRNA 1 and Skp2 shRNA 2 groups were(69?2±5?6)% and(64?5 ±6?1)%; expression of Skp2 protein were reduced(73?5±4?2)%, and(67?3±3?2)%; expression of p27 protein were increased (63?6± 2?2)% and(57?3±2?4)%;expression of p21 protein were increased(54?6 ±3?4)% and(51?7± 3?1)%. Conclusion In?hibiting the expression of Skp2 gene can significantly suppress the growth of transplanted tumor, Skp2 is expected to become a target for gene therapy of lung cancer in future.
